RT Journal Article
T1 Antioxidant and Protective Mechanisms against Hypoxia andHypoglycaemia in Cortical Neurons in Vitro
A1 Merino, José Joaquín
A1 Roncero Romero, César
A1 Oset Gasque, María Jesús
A1 Naddaf, Ahmad
A1 González Prieto, María Pilar
AB In the present work, we have studied whether cell death could be induced in cortical neurons from rats subjected to different period of O2 deprivation and low glucose (ODLG). This “in vitro” model is designed to emulate the penumbra area under ischemia. In these conditions, cortical neurons displayed loss of mitochondrial respiratory ability however, nor necrosis neither apoptosis occurred despite ROS production. The absence of cellular death could be a consequence of increased antioxidant responses such as superoxide dismutase-1 (SOD1) and GPX3. In addition, the levels of reduced glutathione were augmented and HIF-1/3α overexpressed. After long periods of ODLG (12–24 h) cortical neurons showed cellular and mitochondrial membrane alterations and did not recuperate cellular viability during reperfusion. This could mean that therapies directed toward prevention of cellular and mitochondrial membrane imbalance or cell death through mechanisms other than necrosis or apoptosis, like authophagy, may be a way to prevent ODLG damage.
PB MDPI
SN 1422-0067
YR 2014
FD 2014-02-12
LK https://hdl.handle.net/20.500.14352/34802
UL https://hdl.handle.net/20.500.14352/34802
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO UCM- Santander
NO Cienporciennatural
DS Docta Complutense
RD 28 abr 2024