RT Journal Article
T1 Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor
A1 Palacio, Irene
A1 Moreno, Miguel
A1 Náñez, Almudena
A1 Purwidyantri, Agnes
A1 Domingues, Telma
A1 Cabral, Patricia D.
A1 Borme, Jérôme
A1 Marciello, Marzia
A1 Mendieta-Moreno, Jesús Ignacio
A1 Torres-Vázquez, Beatriz
A1 Martínez, José Ignacio
A1 López, María Francisca
A1 García-Hernández, Mar
A1 Vázquez, Luis
A1 Jelínek, Pavel
A1 Alpuim, Pedro
A1 Briones, Carlos
A1 Martín-Gago, José Ángel
AB Biosensors based on graphene field-effect transistors have become a promising tool for detecting a broad range of analytes. However, their performance is substantially affected by the functionalization protocol. In this work, we use a controlled in-vacuum physical method for the covalent functionalization of graphene to construct ultrasensitive aptamer-based biosensors (aptasensors) able to detect hepatitis C virus core protein. These devices are highly specific and robust, achieving attomolar detection of the viral protein in human blood plasma. Such an improved sensitivity is rationalized by theoretical calculations showing that induced polarization at the graphene interface, caused by the proximity of covalently bound molecular probe, modulates the charge balance at the graphene/aptamer interface. This charge balance causes a net shift of the Dirac cone providing enhanced sensitivity for the attomolar detection of the target proteins. Such an unexpected effect paves the way for using this kind of graphene-based functionalized platforms for ultrasensitive and real-time diagnostics of different diseases.
SN 0956-5663
YR 2023
FD 2023-02
LK https://hdl.handle.net/20.500.14352/100703
UL https://hdl.handle.net/20.500.14352/100703
LA eng
NO Palacio I, Moreno M, Náñez A, Purwidyantri A, Domingues T, Cabral PD, et al. Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor. Biosensors and Bioelectronics 2023;222:115006. https://doi.org/10.1016/j.bios.2022.115006.
NO EU Graphene Flagship funding
NO Ministerio de Ciencia e Innovación (España)
NO European Structural Funds
NO Portuguese Foundation for Science and Technology
NO Agencia Estatal de Investigación
NO Centro Superior de Investigaciones Científicas
NO Comunidad de Madrid
NO Fundación "la Caixa"
DS Docta Complutense
RD 5 jul 2025