RT Journal Article
T1 Fipronil sulfone induced higher cytotoxicity than fipronil in SH-SY5Y cells: Protection by antioxidants
A1 Romero Martínez, Manuel Alejandro
A1 Ramos Alonso, Eva
A1 Ares Lombán, Irma
A1 Castellano Santos, Víctor Jesús
A1 Martínez Caballero, Marta
A1 Martínez Larrañaga, María Rosa
A1 Anadón Navarro, Arturo Ramón
A1 Martínez Caballero, María Aranzazu
AB Fipronil is a broad spectrum insecticide from the phenyl pyrazole family, which targets GABA receptor. Limited information is available about the metabolite fipronil sulfone cytotoxic actions. This study examined in vitro neurotoxicity of fipronil and fipronil sulfone and evaluated Trolox (vitamin E analog) (0.3, 1μM), N-acetyl-cysteine (0.5, 1mM), melatonin (0.1, 1μM) and Tempol (superoxide dismutase analog) (0.3, 0.5mM) protective role in SH-SY5Y cells. MTT and LDH assays were carried out to assess the cytotoxicity of fipronil and fipronil sulfone at 3-100μM concentrations. Fipronil sulfone was more toxic than fipronil. Tempol showed the best neuroprotectant profile against fipronil (50 and 150μM) and fipronil sulfone (3 and 10μM) reaching control levels. Fipronil (100μM) and fipronil sulfone (3μM) treatments induced a 4.7- and 5-fold increases in lipid peroxides measured as malondialdehyde (MDA) and a 2.2- and 2.0-fold increases in the levels of nitric oxide (NO). These results suggest that oxidative stress observed may be one of the major mechanisms of fipronil-induced neurotoxicity and it may be attributed in part to fipronil disposition and metabolism. Our results led us postulate that metabolite fipronil sulfone might be responsible for the fipronil-induced toxicity rather than fipronil itself.
PB Elsevier
SN 0378-4274
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/97695
UL https://hdl.handle.net/20.500.14352/97695
LA eng
NO Romero A, Ramos E, Ares I, Castellano V, Martínez M, Martínez-Larrañaga MR, Anadón A, Martínez MA. Fipronil sulfone induced higher cytotoxicity than fipronil in SH-SY5Y cells: Protection by antioxidants. Toxicol Lett. 2016 Jun 11;252:42-9. doi: 10.1016/j.toxlet.2016.04.005. Epub 2016 Apr 8. PMID: 27067106
NO Universidad Complutense de Madrid
NO Comunidad de Madrid
DS Docta Complutense
RD 23 abr 2025