RT Journal Article
T1 Caloric restriction induces heat shock response and inhibits B16F10 cell tumorigenesis both in vitro and in vivo
A1 Garrido Novelle, Marta
A1 Davis, Asheley
A1 Price, Nathan
A1 Ali, Ahmed
A1 Fürer Galvan, Stefanie
A1 Zhang, Yongqing
A1 Becker, Kevin
A1 Bernier, Michel
A1 De Cabo, Rafael
AB Caloric restriction (CR) without malnutrition is one of the most consistent strategies for increasing mean and maximal lifespan and delaying the onset of age-associated diseases. Stress resistance is a common trait of many long-lived mutants and life-extending interventions, including CR. Indeed, better protection against heat shock and other genotoxic insults have helped explain the pro-survival properties of CR. In this study, both in vitro and in vivo responses to heat shock were investigated using two different models of CR. Murine B16F10 melanoma cells treated with serum from CR-fed rats showed lower proliferation, increased tolerance to heat shock and enhanced HSP-70 expression, compared to serum from ad libitum-fed animals. Similar effects were observed in B16F10 cells implanted subcutaneously in male C57BL/6 mice subjected to CR. Microarray analysis identified a number of genes and pathways whose expression profile were similar in both models. These results suggest that the use of an in vitro model could be a good alternative to study the mechanisms by which CR exerts its anti-tumorigenic effects.
PB Impact Journals
SN 1945-4589
YR 2015
FD 2015-04
LK https://hdl.handle.net/20.500.14352/94861
UL https://hdl.handle.net/20.500.14352/94861
LA eng
NO Novelle, Marta G., Ashley Davis, Nathan L. Price, Ahmed Ali, Stefanie Fürer-Galvan, Yongqing Zhang, Kevin Becker, Michel Bernier, y Rafael De Cabo. «Caloric Restriction Induces Heat Shock Response and Inhibits B16F10 Cell Tumorigenesis Both in Vitro and in Vivo». Aging 7, n.o 4 (5 de abril de 2015): 233-40. https://doi.org/10.18632/aging.100732.
NO This work has been supported by the Intramural Research Program of the National Institute on Aging, National Institutes of Health. MGN was supported by CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), an initiative of ISCIII, Spain.
NO Instituto de Salud Carlos III
NO National Institutes of Health
DS Docta Complutense
RD 7 abr 2025