%0 Journal Article
%A Barderas Manchado, Rodrigo
%A Campuzano Ruiz, Susana
%A Manuel De Villena Rueda, Francisco Javier
%A Muñoz San Martín, Cristina
%A Pedrero Muñoz, María
%A Pingarrón Carrazón, José Manuel
%A Domínguez, Gemma
%A Rodríguez, Nuria
%T Disposable Amperometric Immunosensor for theDetermination of the E-Cadherin Tumor SuppressorProtein in Cancer Cells and Human Tissues
%D 2018
%U https://hdl.handle.net/20.500.14352/132190
%X This paper describes the results obtained in the development of the first electrochemical immunosensor described to date for the detection of E-cadherin (E-cad) protein, a relevant biomarker of prognosis and metastasis in cancer, based on the use of magnetic microcarriers (MBs) and amperometric transduction at screen-printed carbon electrodes (SPCEs). Thus, the determination of E- cad protein involved the use of two specific antibodies against this protein (one of them labelled with HRP) in a sandwich configuration onto HOOC-MBs. The magnetic bioconjugates were captured onto SPCEs and the ampero- metric transduction was performed using the H2 O2/hydro- quinone (HQ) system. Under optimal conditions, this bioplatform demonstrated a wide linear concentration range (0.50–25 ng mL 1 ) and a detection limit as low as 0.16 ng mL 1 , well below the optimal cut-off level for the E-cad protein (defined as 10,000 ng mL 1 for soluble E- cad levels in serum). The developed sensor also showed a good reproducibility among measurements with seven different sensors constructed in the same manner (RSD, 5.4 %), stability for more than 15 days and good specificity towards other proteins commonly found on biological samples. The applicability of this simple handling bioplat- form for the direct determination of this protein in cell lysates with different metastatic potential and extracts from paraffined-embedded human colorectal cancer tis- sues of different grade were also demonstrated.
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