RT Journal Article
T1 Extraction and Analysis by HPLC-DAD of Carotenoids in Human Faeces from Spanish Adults
A1 Rodríguez Rodríguez, Elena
A1 Beltrán De Miguel, Beatriz Teresa
A1 Samaniego Aguilar, Kerly X.
A1 Sánchez Prieto, Milagros
A1 Estévez Santiago, Rocío
A1 Olmedilla Alonso, Begoña
AB Carotenoids are bioactive compounds with widely accepted health benefits. Their quantification in human faeces can be a useful non-invasive approach to assess their bioavailability. Identification and quantification of major dietary carotenoids in human faeces was the aim of the present study. Faeces and dietary intake were obtained from 101 healthy adults (45–65 years). Carotenoid concentrations were determined by HPLC in faeces and by 3-day food records in dietary intake. Carotenoids quantified in faeces (μg/g dry weight, median) were: β-carotene (39.5), lycopene (20), lutein (17.5), phytoene (11.4), zeaxanthin (6.3), β-cryptoxanthin (4.5), phytofluene (2.9). α-carotene (5.3) and violaxanthin were found 75.5% and 7.1% of the faeces. The carotenoids found in the highest concentrations corresponded to the ones consumed in the greatest amounts (μg/d): lycopene (13,146), phytoene (2697), β-carotene (1812), lutein+zeaxanthin (1148). Carotenoid concentration in faeces and in dietary intake showed correlation for the total non-provitamin A carotenoids (r = 0.302; p = 0.003), phytoene (r = 0.339; p = 0.001), phytofluene (r = 0.279; p = 0.005), lycopene (0.223; p = 0.027), lutein+zeaxanthin (r = 0.291; p = 0.04) and β-cryptoxanthin (r = 0.323; p = 0.001). A high proportion of dietary carotenoids, especially those with provitamin A activity and some of their isomers, reach the large intestine, suggesting a low bioavailability of their intact forms.
PB MDPI
SN 2076-3921
YR 2020
FD 2020-06-03
LK https://hdl.handle.net/20.500.14352/8309
UL https://hdl.handle.net/20.500.14352/8309
LA eng
NO Rodríguez Rodríguez, E., Beltrán De Miguel, B. T., Samaniego Aguilar, K. X. et al. «Extraction and Analysis by HPLC-DAD of Carotenoids in Human Faeces from Spanish Adults». Antioxidants, vol. 9, n.o 6, junio de 2020, p. 484. DOI.org (Crossref), https://doi.org/10.3390/antiox9060484.
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional
NO Consejo Superior de Investigaciones Científicas (España)
DS Docta Complutense
RD 11 abr 2025