RT Journal Article
T1 Surface activity as a crucial factor of the biological actions of Ole e1, the main aeroallergen of olive tree (Olea europaea) pollen
A1 López Rodríguez, Juan Carlos
A1 Barderas Manchado, Rodrigo
A1 Echaide Torreguitar, Mercedes
A1 Pérez-Gil, Jesús
A1 Villalba, Mayte
A1 Batanero Cremades, Eva
A1 Cruz Rodríguez, Antonio
AB Aeroallergens are airborne substancesmainly proteinscapable of triggering Th2-immune responses in respiratory allergies. They enter into the body through the upper airways, reaching the mucosa afterward. Mucosae lining at the luminal side consists of an epithelial barrier completely covered by mucus and pulmonary surfactant. Both pulmonary surfactant and plasma membrane of the epithelial cells represent two physiological phospholipid-based barriers where allergens first impact before triggering their biological effects. The interaction of allergens with lipids at relevant physiological surfaces could promote structural changes on the molecule, resulting on a potential modification of its allergenic properties. In this work, we have first described the surface and phospholipid interaction capabilities of the clinically relevant aeroallergen Ole e 1, the main allergen of olive tree pollen. By using epifluorescence microscopy of Langmuir transferred films, we observed that lipid-packed ordered domains may function as a preferential location for allergen to accumulate at the air−liquid interface, an effect that is abolished in the presence of cholestenone. The possible implications of phospholipid-interfacial effects in the modification of allergen structural and functional properties will be discussed
PB American Chemical Society
SN 0743-7463, ESSN: 1520-5827
YR 2016
FD 2016-10-25
LK https://hdl.handle.net/20.500.14352/17862
UL https://hdl.handle.net/20.500.14352/17862
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 17 abr 2025