RT Journal Article
T1 Control by potassium of the size distribution of Escherichia coli FtsZ polymers is independent of GTPase activity
A1 Ahijado Guzmán, Rubén
A1 Alfonso, Carlos
A1 Reija, Belén
A1 Salvarelli, Estefanía
A1 Mingorance, Jesús
A1 Zorrilla, Silvia
A1 Monterroso, Begoña
A1 Rivas, Germán
AB The influence of potassium content (at neutral pH and millimolar Mg(2+)) on the size distribution of FtsZ polymers formed in the presence of constantly replenished GTP under steady-state conditions was studied by a combination of biophysical methods. The size of the GTP-FtsZ polymers decreased with lower potassium concentration, in contrast with the increase in the mass of the GDP-FtsZ oligomers, whereas no effect was observed on FtsZ GTPase activity and critical concentration of polymerization. Remarkably, the concerted formation of a narrow size distribution of GTP-FtsZ polymers previously observed at high salt concentration was maintained in all KCl concentrations tested. Polymers induced with guanosine 5'-(¿,ß-methylene)triphosphate, a slowly hydrolyzable analog of GTP, became larger and polydisperse as the potassium concentration was decreased. Our results suggest that the potassium dependence of the GTP-FtsZ polymer size may be related to changes in the subunit turnover rate that are independent of the GTP hydrolysis rate. The formation of a narrow size distribution of FtsZ polymers under very different solution conditions indicates that it is an inherent feature of FtsZ, not observed in other filament-forming proteins, with potential implications in the structural organization of the functional Z-ring
PB Elsevier
SN 0021-9258
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/92313
UL https://hdl.handle.net/20.500.14352/92313
LA eng
NO Ahijado-Guzmán, Rubén, et al. «Control by Potassium of the Size Distribution of Escherichia Coli FtsZ Polymers Is Independent of GTPase Activity». Journal of Biological Chemistry, vol. 288, n.o 38, septiembre de 2013, pp. 27358-65.  https://doi.org/10.1074/jbc.M113.482943.
DS Docta Complutense
RD 6 abr 2025