RT Journal Article
T1 Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
A1 García Benzaquén, Nerea
A1 Marta Hernández, 
A1 Maialen Gutierrez-Boada, 
A1 Antonio Valero, 
A1 Navarro Gómez, Alejandro
A1 Milagros Muñoz-Chimeno, 
A1 Fernández Manzano, Álvaro
A1 Franco Matías Escobar, 
A1 Martínez Alares, Irene
A1 Bárcena Asensio, María Carmen
A1 González Domínguez, Sergio
A1 Ana Avellón, 
A1 Jose M. Eiros, 
A1 Gislaine Fongaro, 
A1 Domínguez Rodríguez, Lucas José
A1 Goyache Goñi, Joaquín
A1 David Rodríguez-Lázaro, 
AB Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription realtime PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.
PB FRONTIERS MEDIA SA
SN 2297-1769
YR 2020
FD 2020-01-28
LK https://hdl.handle.net/20.500.14352/93121
UL https://hdl.handle.net/20.500.14352/93121
LA eng
NO García N, Hernández M, Gutierrez-Boada M, Valero A, Navarro A, Muñoz-Chimeno M, Fernández-Manzano A, Escobar FM, Martínez I, Bárcena C, González S, Avellón A, Eiros JM, Fongaro G, Domínguez L, Goyache J and Rodríguez-Lázaro D (2020) Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017. Front. Microbiol. 10:2990. doi: 10.3389/fmicb.2019.02990
NO This study was partially supported by the RTA2014-00024-C04 from the Spanish Ministry of Economy and Innovation.
NO Ministerio de Ciencia e Innovación (RTA2014-00024-C04)
DS Docta Complutense
RD 4 ago 2024