RT Journal Article T1 Vegetable Oils Rich in Polyunsaturated Fatty Acids Supplementation of Dairy Cows’ Diets: Effects on Productive and Reproductive Performance A1 Castro Madrigal, Teresa A1 Martinez, Diego A1 Isabel Redondo, Beatriz A1 Cabezas Albéniz, Almudena A1 Jimeno, Vicente AB The aim of this study was to determine how polyunsaturated fatty acids (PUFA) supplementation can affect the productive and reproductive performance in dairy cows subjected to a fixed-time artificial insemination (TAI) protocol under farm conditions. One hundred and ninety-eight Holstein non-pregnant cows were used. Treatments consisted of a control diet (CON), without added oil, and two diets supplemented with either 2.3% soybean oil (SOY) or 2.3% linseed oil (LIN) as dry matter. The diets were formulated to be isoenergetic and isoproteic. Dry matter intake and milk yield were similar among treatments (p > 0.05). Both the percentage of fat (p = 0.011) and protein (p = 0.022) were higher in milk from animals not fed with oil (CON). The greatest saturated fatty acid (SFA) concentration (p < 0.0001) was observed in milk from cows fed the control diet, without added oil. The monounsaturated fatty acids (MUFA), PUFA, and the n-3 PUFA content was higher (p < 0.0001) in the milk from animals fed with oil with respect to the control treatment. The C18:2 cis-9, trans-11 in the milk of animals fed with oil supplements was significantly higher (p < 0.0001) than in that of the control group. Animals supplemented with linseed oil tended to show higher plasma progesterone level (p = 0.09) and a higher number of pregnant cows on the first artificial insemination (p = 0.07). These animals tended to reduce the number of TAI (p = 0.08). In brief, results showed that vegetable oils rich in PUFA supplementation considerably improve the nutritional profile of milk. PUFA n-3 supplementation slightly improves some reproductive parameters in dairy cows subjected to the fixed-time artificial insemination (TAI) protocol. PB MDPI SN 2076-2615 YR 2019 FD 2019-04-30 LK https://hdl.handle.net/20.500.14352/12500 UL https://hdl.handle.net/20.500.14352/12500 LA eng NO Centro para el Desarrollo Tecnológico Industrial (CDTI) DS Docta Complutense RD 3 may 2024