RT Journal Article
T1 Urinary excretion of parathyroid hormone-related protein correlates with renal function in control rats and rats with cisplatin nephrotoxicity
A1 Ortega, Arantxa
A1 Olea Herrero, Nuria
A1 Arenas, María Isabel
A1 Vélez Vélez, Esperanza
A1 Moreno Gómez Toledano, Rafael
A1 Múñoz Moreno, Carmen
A1 Lázaro Fernández, Alberto
A1 Esbrit, Pedro
A1 Tejedor Jorge, Alberto
A1 Bosch, Ricardo J.
AB Parathyroid hormone-related protein (PTHrP) and its receptor are abundantly expressed throughout the renal parenchyma, where PTHrP exerts a modulatory action on renal function. PTHrP upregulation is a common event associated with the mechanism of renal injury and repair. However, no study has yet explored the putative excretion of PTHrP in urine, including its potential relationship with renal function. In the present study, we tested this hypothesis by studying the well-known rat model of acute renal injury induced by the chemotherapeutic agent cisplatin. Using Western blot analysis, we could detect a single protein band, corresponding to intact PTHrP, in the urine of both control and cisplatin-injected rats, whose levels were significantly higher in the latter group. PTHrP was detected in rat urine by dot blot, and its quantification with two specific ELISA kits showed that, compared with control rats, those treated with cisplatin displayed a significant increase in urinary PTHrP (expressed as the PTHrP-to-creatinine ratio or 24-h excretion). In addition, a positive correlation between urinary PTHrP excretion and serum creatinine was found in these animals. In conclusion, our data demonstrate that PTHrP is excreted in rat urine and that this excretion is higher with the decrease of renal function. This suggests that urinary PTHrP levels might be a renal function marker.
PB American Physiological Society
SN 1931-857X
YR 2019
FD 2019-09-25
LK https://hdl.handle.net/20.500.14352/116052
UL https://hdl.handle.net/20.500.14352/116052
LA eng
NO Ortega A, Olea-Herrero N, Arenas MI, Vélez-Vélez E, Moreno-Gómez-Toledano R, Muñoz-Moreno C, Lázaro A, Esbrit P, Tejedor A, Bosch RJ. Urinary excretion of parathyroid hormone-related protein correlates with renal function in control rats and rats with cisplatin nephrotoxicity. Am J Physiol Renal Physiol. 2019 Oct 1;317(4):F874-F880
NO Instituto de Salud Carlos III
NO Unión Europea
DS Docta Complutense
RD 18 abr 2026