RT Journal Article
T1 Cirrhosis decreases vasoconstrictor response to electrical field stimulation in rat mesenteric artery: role of calcitonin gene-related peptide
A1 Blanco Rivero, Javier
A1 Márquez Rodas, Iván
A1 Sastre, Esther
A1 Cogolludo Torralba, Ángel Luis
A1 Pérez Vizcaíno, Francisco
A1 Del Campo Milán, Lara
A1 Nava Hidalgo, María Paz
A1 Balfagón, Gloria
AB Our study determines alterations in the vasoconstrictor response elicited by electric field stimulation (EFS) in mesenteric arteries from cirrhotic rats treated with CCl(4), and how calcitonin gene-related peptide (CGRP) participates in this response. Vasoconstriction induced by EFS was analysed in the absence and presence of the CGRP receptor antagonist CGRP(8-37) in arterial segments from control and cirrhotic rats. The vasodilator response to exogenous CGRP was tested in both groups of rats, and the interference of the guanylate cyclase inhibitor ODQ or the K(ATP) channel blocker glibenclamide was analysed only in segments from cirrhotic rats. The vasodilator response to the K(ATP) channel opener pinacidil and to 8-bromo-cyclic GMP was tested. The K(ATP) currents were recorded using the patch-clamp technique. Expression of receptor activity-modifying protein 1 (RAMP1), calcitonin receptor-like receptor, Kir 6.1 and sulfonylurea receptor 2B (SUR2B) was also analysed. Release of CGRP and cGMP was measured. The EFS-elicited vasoconstriction was less in segments from cirrhotic rats. The presence of CGRP(8-37) increased the EFS-induced response only in segments from cirrhotic rats. The CGRP-induced vasodilatation was greater in segments from cirrhotic rats, and was inhibited by ODQ or glibenclamide. Both pinacidil and 8-bromo-cyclic GMP induced a stronger vasodilator response in segments from cirrhotic rats. Pinacidil induced greater K(ATP) currents in cirrhotic myocytes. Expression of RAMP1, calcitonin receptor-like receptor, Kir 6.1 and SUR2B was not modified by liver cirrhosis. Liver cirrhosis increased CGRP release, but did not modify cGMP formation. The decreased vasoconstrictor response to EFS in cirrhosis is mediated by increased vasodilator response to CGRP, as well as increased K(ATP) channel gating. This effect of CGRP may play a role in the splanchnic vasodilatation present in liver cirrhosis.
PB Wiley
SN 0958-0670
YR 2011
FD 2011-01-19
LK https://hdl.handle.net/20.500.14352/95614
UL https://hdl.handle.net/20.500.14352/95614
LA eng
NO Blanco-Rivero J, Márquez-Rodas I, Sastre E, Cogolludo A, Pérez-Vizcaíno F, del Campo L, Nava MP, Balfagón G. Cirrhosis decreases vasoconstrictor response to electrical field stimulation in rat mesenteric artery: role of calcitonin gene-related peptide. Exp Physiol. 2011 Mar;96(3):275-86.
NO Instituto de Salud Carlos III (España)
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 8 abr 2025