RT Journal Article
T1 Hypoxia induces expression of the chemokines monocyte chemoattractant protein-1 (MCP-1) and IL-8 in human dermal fibroblasts
A1 Galindo Izquierdo, María
A1 Santiago, Begoña
A1 Alcami, Jose
A1 Rivero, M.
A1 Martín Serrano, J.
A1 Pablos Álvarez, José Luis
AB Hypoxia is an important factor in the pathophysiology of vascular and inflammatory diseases. Leucocyte infiltration, as a consequence of adhesion molecule up-regulation and chemokine release, is a prominent feature of these diseases. The objective of our study was to investigate the potential role of resident fibroblasts in hypoxia-induced chemotactic responses. We show that MCP-1 and IL-8 mRNA are specifically induced by hypoxia in dermal fibroblasts. This response is paralleled by increased NF-kappaB p65/p50 binding activity, and it is inhibited by pretreatment with N-acetyl-L-cysteine. MCP-1 secreted by fibroblasts is chemotactic for monocytic cells and this activity is significantly increased by hypoxia. Chemotactic index correlates with MCP-1 protein levels and is significantly decreased by neutralizing anti-MCP-1 MoAb. These findings demonstrate the ability of resident fibroblasts to mediate chemotaxis of leucocytes through the release of chemokines in response to hypoxia. Our data point to MCP-1 as an important component in this response, and therefore it may be a potential target in inflammatory responses associated with hypoxia.
PB Oxford University Press
SN 0009-9104
YR 2001
FD 2001
LK https://hdl.handle.net/20.500.14352/114212
UL https://hdl.handle.net/20.500.14352/114212
LA eng
NO Galindo M, Santiago B, Alcami J, Rivero M, Martín-Serrano J, Pablos JL. Hypoxia induces expression of the chemokines monocyte chemoattractant protein-1 (MCP-1) and IL-8 in human dermal fibroblasts. Clin Exp Immunol. 2001 Jan;123(1):36-41.
NO Ministerio de Educación y Cultura (España)
NO Instituto de Salud Carlos III (España)
DS Docta Complutense
RD 14 dic 2025