RT Journal Article
T1 HPLC isolation of antioxidant constituents from Xanthoparmelia spp
A1 Amo De Paz, Guillermo
A1 Raggio Quílez, José
A1 Gómez-Serranillos Cuadrado, María Pilar
A1 Palomino Ruiz-Poveda, Olga María
A1 González Burgos, Elena María
A1 Carretero Accame, María Emilia
A1 Crespo De Las Casas, Ana María
AB A chromatographic method is described for the purification and characterization of secondary lichen substances with biological activity. A simple reversed-phase high-performance liquid chromatography method with gradient elution has been developed that allows the determination and isolation of salazinic, usnic and stictic acids from lichen samples in a single run and the quantification of every acid in the tested extracts. The antioxidant activity of both the isolated compounds and the respective lichen belonging to Xanthoparmelia genus was determined by the Oxygen Radical Absorbance Capacity (ORAC) assay; their effect as free radical scavengers, effect on cell survival by the 3(4,5-dimethyltiazol-2-yl)-2,5-diphenyltetrazolium reduction assay and 2′,7′-dichlorofluorescin diacetate method were tested on U373 MG human astrocytome cell line. Both lichens extracts and all isolated compounds protected U373 MG cells from hydrogen peroxide-induced damage, suggesting that they could act as antioxidant agents in those neurodegenerative disorders associated with oxidative damage, such as Alzheimer's disease and Parkinson's disease.
PB Elsevier
SN 0731-7085
YR 2010
FD 2010-04-24
LK https://hdl.handle.net/20.500.14352/92680
UL https://hdl.handle.net/20.500.14352/92680
LA eng
NO Amo de Paz, G.; Raggio, J.; Gómez-Serranillos, M.P.; Palomino, O.M.; González-Burgos, E.; Carretero, M.E.; Crespo, A. HPLC isolation of antioxidant constituents from Xanthoparmelia spp. J. Pharm. Biomed. Anal. 2010, 53, 165–171
DS Docta Complutense
RD 6 oct 2024