RT Journal Article
T1 Oligomerization of Sticholysins from Förster Resonance Energy Transfer
A1 Palacios-Ortega, Juan
A1 Rivera de la Torre, Esperanza
A1 García-Linares, Sara
A1 Gavilanes, José G.
A1 Martínez Del Pozo, Álvaro
A1 Slotte, J. Peter
AB Sticholysins are pore-forming toxins produced by sea anemones that are members of the actinoporin family. They exert their activity by forming pores on membranes, provided they have sphingomyelin. To assemble into pores, specific recognition, binding, and oligomerization are required. While recognition and binding have been extensively studied, delving into the oligomerization process and the stoichiometry of the pores has been more difficult. Here, we present evidence that these toxins are capable of oligomerizing in solution and suggesting that the interaction of sticholysin II (StnII) with its isoform sticholysin I (StnI) is stronger than that of StnI with itself. We also show that the stoichiometry of the final, thermodynamically stable StnI pores is, at least, heptameric. Furthermore, our results indicate that this association maintains its oligomerization number when StnII is included, indicating that the stoichiometry of StnII is also of that order, and not tetrameric, as previously thought. These results are compatible with the stoichiometry observed for the crystallized pore of FraC, another very similar actinoporin produced by a different sea anemone species. Our results also indicate that the stoichiometry of actinoporin pores in equilibrium is conserved regardless of the particular composition of a given pore ensemble, which we have shown for mixed sticholysin pores.
PB American Chemical Society
SN 0006-2960; Electronic: 1520-4995
YR 2021
FD 2021-01-14
LK https://hdl.handle.net/20.500.14352/8070
UL https://hdl.handle.net/20.500.14352/8070
LA eng
NO Universidad Complutense de Madrid/Banco de Santander
NO Sigrid Juselius Foundation
NO Jane and Aatos Erkko Foundation
DS Docta Complutense
RD 25 abr 2025