RT Journal Article
T1 Six month delivery of GDNF from PLGA/vitamin E biodegradable microspheres after intravitreal injection in rabbits
A1 García Caballero, Cristina G.
A1 Prieto Calvo, Esther
A1 Checa Casalengua, Patricia
A1 García Martín, Elena Salobrar
A1 Polo Llorens, Vicente
A1 García Feijoo, Julián
A1 Molina Martínez, Irene Teresa
A1 Bravo Osuna, Irene
A1 Herrero Vanrell, María Del Rocío
AB Local long-term delivery of glial cell line derived neurotrophic factor (GDNF) from vitamin E/poly-lactic-co-glycolic acid microspheres (MSs) protects retinal ganglion cells in an animal model of glaucoma for up to 11 weeks. However, the pharmacokinetics of GDNF after intravitreal injection of MSs is not known. We evaluated the GDNF levels after a single intravitreal injection of GDNF/VitE MSs. Biodegradable MSs were prepared by the solid-oil-in-water emulsion-solvent evaporation technique and characterized. Rabbits received a single intravitreal injection (50 μL) of GDNF/VitE MSs (4%w/v; 24 right eyes; 74.85 ng GDNF), blank MSs (4%w/v; 24 left eyes), and balanced salt solution (4 eyes). Two controls eyes received no injections. At 24 hours, 1, 4, 6, 8, 12, 18, and 24 weeks after injection, the eyes were enucleated, and the intravitreal GDNF levels were quantified. Pharmacokinetic data were analysed according to non-compartmental model. Intraocular GDNF levels of 717.1 ± 145.1 pg/mL were observed at 24 hours for GDNF-loaded MSs, followed by a plateau (745.3 ± 25.5 pg/mL) until day 28. After that, a second plateau (17.4 ± 3.7 pg/mL) occurred from 8 to 24 weeks postinjection, significantly higher than the basal levels. Eyes injected with GDNF/vitE and Blank-MSs did not show any abnormalities during the six-months follow up after administration. The single injection of GDNF/VitE MSs provided a sustained controlled release of the neurotrophic factor in a controlled fashion for up to six months.
PB Elsevier
SN 0928-0987
YR 2017
FD 2017-05-30
LK https://hdl.handle.net/20.500.14352/18740
UL https://hdl.handle.net/20.500.14352/18740
LA eng
NO García Caballero, C. G., Prieto Calvo, E., Checa Casalengua, P. et al. «Six Month Delivery of GDNF from PLGA/Vitamin E Biodegradable Microspheres after Intravitreal Injection in Rabbits». European Journal of Pharmaceutical Sciences, vol. 103, mayo de 2017, pp. 19-26. DOI.org (Crossref), https://doi.org/10.1016/j.ejps.2017.02.037.
NO Ministerio de Economía, Comercio y Empresa (España)/Fondo Europeo de Desarrollo Regional
NO Instituto de Salud Carlos III
NO Redes Temáticas de Investigación Cooperativa en Salud (España)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 5 abr 2025