RT Journal Article
T1 Biofilm formation on dental implants with different surface micro‐topography: an in vitro study
A1 Bermejo, Patricia
A1 Sánchez Beltrán, María Del Carmen
A1 Llama Palacios, María Arantxazu
A1 Figuero Ruiz, Elena
A1 Herrera González, David
A1 Sanz Alonso, Mariano
AB ObjectivesTo compare biofilm formation on whole dental titanium implants with different surface micro‐topography.MethodsA multispecies in vitro biofilm model consisting of initial (Streptococcus oralis and Actinomyces naeslundii), early (Veillonella parvula), secondary (Fusobacterium nucleatum) and late colonizers (Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans) was grown for 96 hr on sterile titanium dental implants with either minimal (Sa: 0.5–1.0 mm) or moderate‐roughness titanium surfaces (Sa: 1.1–2.0 mm). The resulting biofilms were studied with Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscope. Concentrations (colony‐forming units per mL [CFU/ml]) of each bacterium were measured by quantitative Polymerase Chain Reaction (qPCR) and compared by Student t tests.ResultsA biofilm, located mainly at the peak and lateral areas of the implant threads, was observed on both implant surfaces, with a greater biomass and a greater live/dead ratio in moderate‐ compared to minimal‐roughness surface implants. Statistically significant higher values of total bacteria (mean difference = 2.61 × 107 CFU/ml; 95% confidence interval — CI [1.91 × 106; 5.02 × 107]; p = 0.036), F. Nucleatum (mean difference = 4.43 × 106 CFU/ml; 95% CI [1.06 × 106; 7.80 × 106]; p = 0.013) and A. actinomycetemcomitans (mean difference = 2.55 × 107 CFU/ml; 95% CI [1.07 × 107; 4.04 × 107]; p = 0.002), were found in the moderate‐ compared to minimal‐roughness surface dental implants.ConclusionsImplants with moderate‐roughness surfaces accumulated more bacterial biomass and significant higher number of pathogenic bacteria (F. nucleatum and A. actinomycetemcomitans), when compared to implants with minimal‐roughness surfaces, within a similar biofilm structure.
PB Wiley
SN 0905-7161
YR 2019
FD 2019-05-22
LK https://hdl.handle.net/20.500.14352/114665
UL https://hdl.handle.net/20.500.14352/114665
LA eng
NO Bermejo P, Sánchez MC, Llama-Palacios A, Figuero E, Herrera D, Sanz Alonso M. Biofilm formation on dental implants with different surface micro-topography: An in vitro study. Clin Oral Implants Res. 2019 Aug;30(8):725-734. doi: 10.1111/clr.13455
DS Docta Complutense
RD 17 abr 2025