TY  - JOUR
AU  - Largo-de la Torre, Andrea
AU  - Velasco Jiménez, Natalia
AU  - Ortega Mora, Luis Miguel
AU  - Regidor-Cerrillo, Javier
PY  - 2026
DO  - 10.1038/s41598-026-43786-0
UR  - https://hdl.handle.net/20.500.14352/136774
T2  - Scientific Reports
AB  - Toxoplasmosis is a major foodborne zoonosis causing high global disease burden and economic losses in sheep and goat industries. The gold standard for detecting viable Toxoplasma gondii is the mouse bioassay, which involves inoculating tissues from...
LA  - eng
M2  - 1
PB  - Nature Research
KW  - Toxoplasma gondii detection
KW  - 3Rs
KW  - Messenger RNA quantification
KW  - Mouse bioassay
KW  - Parasite viability
KW  - Reverse transcriptase quantitative PCR
TI  - Development and validation of a gra1–bag1 RT-qPCR assay as an alternative to the mouse bioassay for assessing Toxoplasma gondii viability
TY  - journal article
VL  - 16(1)
ER  -