RT Journal Article
T1 A combined analytical-chemometric approach for the in vitro determination of polyphenol bioaccessibility by simulated gastrointestinal digestion
A1 Gómez Mejía, Esther
A1 Rosales Conrado, Noelia
A1 León González, María Eugenia de
A1 Valverde De La Fuente, Alejandro
A1 Madrid Albarrán, María Yolanda
AB In this study, an integrated characterisation through polyphenol and cafeine content and antioxidant activity was combined with chemometric analysis to assess the efects of simulated in vitro gastrointestinal digestion on the bioaccessibility of these bioactive compounds from nine diferent tea infusions. Tea infusions were characterised based on total favonoids, total polyphenols and antioxidant activity, together with the determination of individual polyphenol content. Fourteen phenolic compounds, including phenolic acids, stilbenes and favonoids, were selected based on their reported bioactivity and high accessibility, attributed to their low molecular weight. Both polyphenols and cafeine were initially monitored in raw tea infusions and through the diferent digestion stages (salivary, gastric and duodenal) by capillary high performance liquid chromatography coupled to diode array detection (cHPLC-DAD) and/or HPLC coupled to a triple quadrupole mass analyser (HPLC–MS/MS). Multivariate analysis of the studied bioactives, using principal component analysis and cluster analysis, revealed that the decafeination process seems to increase the stability and concentration of the compounds evaluated during digestion. The greatest transformations occurred mainly in the gastric and duodenal stages, where low bioactivity indices (IVBA) were shown for resveratrol and cafeic acid (IVBA=0%). In contrast, the polyphenols gallic acid, chlorogenic acid and quercetin gave rise to their availability in white, green and oolong infusion teas (IVBA>90%). Furthermore, highly fermented black and pu-erh varieties could be designated as less bioaccessible environments in the duodenum with respect to the tested compounds.
PB Springer
SN 1618-2642
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/71576
UL https://hdl.handle.net/20.500.14352/71576
LA eng
NO CRUE-CSIC (Acuerdos Transformativos 2022)
NO Ministerio de Ciencia e Innovación (MICINN)
NO Comunidad de Madrid/FEDER
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 17 abr 2025