RT Journal Article
T1 New insights into the allergenicity of tropomyosin: a bioinformatics approach
A1 González Fernández, Juan
A1 Rodero Martínez, Marta
A1 Daschner, Álvaro
A1 Cuéllar Del Hoyo, María Del Carmen
AB The invertebrate panallergen tropomyosin is a protein with an extremely simple folding. This makes it a perfect target for investigating structural differences between invertebrate and vertebrate tropomyosins, which are not considered allergenic. Phylogenetic and sequence analyses were conducted in order to explore the differences in primary structure between several tropomyosins and to promote an experimental development in the field of food allergy, based on the study of tropomyosin. The phylogenetic analyses showed that tropomyosin is a useful evolutionary marker. The phylogenetic trees obtained with tropomyosin were not always phylogenetically correct, but they might be useful for allergen avoidance by tropomyosin allergic individuals. Sequence analyses revealed that the probability of alpha helix folding in invertebrate tropomyosins was lower than in all the studied vertebrate ones, except for the Atlantic bluefin tuna Thunnus thynnus tropomyosin. This suggested that the lack of alpha helix folding may be involved in the immunogenicity of tropomyosins. More specifically, the regions adjacent to the positions 133-135 and 201 of the invertebrate tropomyosins, presented lower probability of alpha helix folding than those of vertebrates and are candidates to be responsible for their allergenicity.
PB Springer
SN 0301-4851
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/92457
UL https://hdl.handle.net/20.500.14352/92457
LA eng
NO González-Fernández, J., Rodero, M., Daschner, A. et al. New insights into the allergenicity of tropomyosin: a bioinformatics approach. Mol Biol Rep 41, 6509–6517 (2014). https://doi.org/10.1007/s11033-014-3534-6
NO Fundación Ramón Areces
DS Docta Complutense
RD 30 dic 2025