RT Journal Article
T1 Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages
A1 Bartolomé-Martín, David
A1 Ramírez-Franco, Jorge
A1 Castro López-Tarruella, Enrique
A1 Sánchez-Prieto Borja, José
A1 Torres Molina, Magdalena Isabel
AB Following the exocytosis of neurotransmitter-containing synaptic vesicles, endocytosis is fundamental to re-establishing conditions for synaptic transmission. As there are distinct endocytotic pathways that each differ in their efficiency to generate releasable synaptic vesicles, we used the dye FM1-43 to track vesicle recycling, and to determine whether nerve terminals use multiple pathways of endocytosis. We identified two types of synaptic boutons in cultured cerebellar granule cells that were characterized by weak or strong FM1-43-unloading profiles. Decreasing the extent of exocytosis dramatically increased the proportion of synaptic boutons that exhibited strong FM1-43-unloading and dramatically reduced the number of endosome-like structures. Hence, we concluded that efficient recycling of synaptic vesicles is concomitant with the formation of non-releasable endosomes in both types of synaptic boutons, although to different extents. Furthermore, cell maturation in culture increased the proportion of synaptic boutons that were capable of an intense release response, whereas the chronic blockage of synaptic activity diminished the capacity of boutons to release dye.
PB The company of Biologists
SN 0021-9533
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/129658
UL https://hdl.handle.net/20.500.14352/129658
LA eng
NO Bartolomé-Martín D, Ramírez-Franco J, Castro E, Sánchez-Prieto J, Torres M. Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages. J Cell Sci. 2012 Jan 15;125(Pt 2):422-34.
NO Ministerio de Educación y Ciencia (España)
NO Instituto de Salud Carlos III
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 21 ene 2026