RT Journal Article
T1 Immunoproteomic analysis of the protective response obtained from vaccination with Candida albicans ecm33 cell wall mutant in mice
A1 Martínez López, Raquel María
A1 Nombela Cano, César
A1 Díez Orejas, Rosalía María
A1 Monteoliva Díaz, Lucía
A1 Gil García, Concepción
AB Systemic candidiasis remains a major cause of disease and death, particularly among immunocompromised patients. The cell wall of Candida albicans defines the interface between host and pathogen and surface proteins are major elicitors of host immune responses during candidiasis. The C. albicans ecm33 mutant (RML2U) presents an altered cell wall, which entails an increase in the outermost protein layer. Vaccination of BALB/c mice with RML2U mutant protected them from a subsequent lethal infection with virulent strain SC5314 in a systemic candidiasis model. Using immunoproteomics (2-DE followed by Immunoblotting) we detected 29 immunoreactive proteins specifically recognized by antibodies from vaccinated mice sera, six of which are described as immunogenic for the first time (Gnd1p, Cit1p, Rpl10Ep, Yst1p, Cys4p, Efb1p). Furthermore, identification of wild type and mutant cell surface proteome (surfome), confirmed us that the mutant surfome presented a larger number of proteins than the wild type. Interestingly, proteins exclusively identified in the mutant surfome (Met6p, Eft2p, Tkl1p, Rpl10Ep, Atp1p, Atp2p) were also detected as immunogenic, supporting the idea that their surface location enhances their immunoprotective capacity.
PB Wiley
SN 1615-9853
YR 2008
FD 2008-07-08
LK https://hdl.handle.net/20.500.14352/95436
UL https://hdl.handle.net/20.500.14352/95436
LA eng
NO Martínez‐López R, Nombela C, Diez‐Orejas R, Monteoliva L, Gil C. Immunoproteomic analysis of the protective response obtained from vaccination with Candida albicans ecm33 cell wall mutant in mice. Proteomics 2008;8:2651–64. https://doi.org/10.1002/pmic.200701056.
NO Comunidad Autónoma de Madrid
NO Universidad Complutense de Madrid
NO Comisión Interministerial de Ciencia y Tecnología
DS Docta Complutense
RD 10 abr 2025