RT Journal Article
T1 Electrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Pellicanò, Alessandro
A1 Campuzano Ruiz, Susana
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Reviejo García, Ángel Julio
A1 Cosio, Maria Stella
A1 Pingarrón Carrazón, José Manuel
AB A highly sensitive disposable amperometric magnetoimmunosensor for the rapid determination of Ara h 2 protein, one of the major peanut allergens, is reported. The approach uses a sandwich configuration involving selective capture and detector antibodies and carboxylic acid-modified magnetic beads (HOOC-MBs). Detector antibodies are labeled with HRP-conjugated secondary antibodies and the MBs bearing the immunoconjugates are magnetically captured on surface of a disposable screen-printed carbon electrode (SPCE). The affinity reactions are monitored amperometrically at −0.20 V (vs a Ag pseudo-reference electrode) in the presence of hydroquinone (HQ) as electron transfer mediator and upon addition of hydrogen peroxide as the enzyme substrate. The developed magnetoimmunosensor exhibits a wide range of linearity between 87 and 10,000 pg/mL Ara h 2 with a detection limit of 26 pg/mL as well as a great selectivity against other non-target proteins. The magnetoimmunosensing platform was successfully applied for the detection of Ara h 2 in different food extracts. After an appropriate sample dilution no matrix effects were observable. The developed methodology was able to detect trace amounts of the peanut allergen (0.0005% or 5.0 mg/kg) in wheat flour spiked samples. The results correlated properly with those provided by a commercial ELISA kit.
PB Elsevier Science 
SN 0925-4005
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/94995
UL https://hdl.handle.net/20.500.14352/94995
LA eng
NO Montiel, V. R. V., Pellicano, A., Campuzano, S., Torrente-Rodríguez, R. M., Reviejo, Á. J., Cosio, M. S., & Pingarrón, J. M. (2016). Electrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2. Sensors and Actuators B: Chemical, 236, 825-833.
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
NO Ministry of Education (Italy)
DS Docta Complutense
RD 20 ago 2024