RT Journal Article
T1 Hybrid Collagenase Nanocapsules for Enhanced Nanocarrier Penetration in Tumoral Tissues
A1 Villegas Díaz, María Rocío
A1 Baeza, Alejandro
A1 Vallet Regí, María Dulce Nombre
AB Poor penetration of drug delivery nanocarriers within dense extracellular matrices constitutes one of the main liabilities of current nanomedicines. The conjugation of proteolytic enzymes on the nanoparticle surface constitutes an attractive alternative. However, the scarce resistance of these enzymes against the action of proteases or other aggressive agents present in the bloodstream strongly limits their application. Herein, a novel nanodevice able to transport proteolytic enzymes coated with an engineered pH-responsive polymeric is presented. This degradable coat protects the housed enzymes against proteolytic attack at the same time that it triggers their release under mild acidic conditions, usually present in many tumoral tissues. These enzyme nanocapsules have been attached on the surface of mesoporous silica nanoparticles, as nanocarrier model, showing a significatively higher penetration of the nanopartides within 3D collagen matrices which housed human osteosarcoma cells (HOS). This strategy can improve the therapeutic efficacy of the current nanomedicines, allowing a more homogeneous and deeper distribution of the therapeutic nanosystems in cancerous tissues.
PB American Chemical Society
SN 1944-8244
YR 2015
FD 2015-11-04
LK https://hdl.handle.net/20.500.14352/23171
UL https://hdl.handle.net/20.500.14352/23171
LA spa
NO ORCID 0000-0002-9042-8865 (Alejandro Baeza)RESEARCHER ID K-8193-2014  (Alejandro Baeza)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Ministerio de Economía y Competitividad (MINECO)
NO Agening Network of Excellence
DS Docta Complutense
RD 4 may 2024