RT Journal Article
T1 EuroPineDB: a high-coverage web database for maritime pine transcriptome
A1 Fernández-Pozo, Noé
A1 Canales, Javier
A1 Guerrero-Fernández, Darío
A1 Villalobos, David P.
A1 Díaz-Moreno, Sara M.
A1 Bautista, Rocío
A1 Flores-Monterroso, Arantxa
A1 Guevara, Ángeles
A1 Perdiguero Jiménez, Pedro
A1 Collada, Carmen
A1 Cervera, Teresa
A1 Soto, Álvaro
A1 Ordás, Ricardo
A1 Cantón, Francisco
A1 Ávila, Concepción
A1 Cánovas, Francisco
A1 Claros, Gonzalo
AB Pinus pinaster is an economically and ecologically important species that is becoming a woody gymnosperm model. Its enormous genome size makes whole-genome sequencing approaches are hard to apply. Therefore, the expressed portion of the genome has to be characterised and the results and annotations have to be stored in dedicated databases.Description: EuroPineDB is the largest sequence collection available for a single pine species, Pinus pinaster (maritime pine), since it comprises 951 641 raw sequence reads obtained from non-normalised cDNA libraries and high-throughput sequencing from adult (xylem, phloem, roots, stem, needles, cones, strobili) and embryonic (germinated embryos, buds, callus) maritime pine tissues. Using open-source tools, sequences were optimally pre-processed, assembled, and extensively annotated (GO, EC and KEGG terms, descriptions, SNPs, SSRs, ORFs and InterPro codes). As a result, a 10.5× P. pinaster genome was covered and assembled in 55 322 UniGenes. A total of 32 919 (59.5%) of P. pinaster UniGenes were annotated with at least one description, revealing at least 18 466 different genes. The complete database, which is designed to be scalable, maintainable, and expandable, is freely available at: http://www.scbi.uma.es/pindb/. It can be retrieved by gene libraries, pine species, annotations, UniGenes and microarrays (i.e., the sequences are distributed in two-colour microarrays; this is the only conifer database that provides this information) and will be periodically updated. Small assemblies can be viewed using a dedicated visualisation tool that connects them with SNPs. Any sequence or annotation set shown on-screen can be downloaded. Retrieval mechanisms for sequences and gene annotations are provided.Conclusions: The EuroPineDB with its integrated information can be used to reveal new knowledge, offers an easy-to-use collection of information to directly support experimental work (including microarray hybridisation), and provides deeper knowledge on the maritime pine transcriptome
PB Springer Nature
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/96697
UL https://hdl.handle.net/20.500.14352/96697
LA eng
NO Fernández-Pozo, N., Canales, J., Guerrero-Fernández, D. et al. EuroPineDB: a high-coverage web database for maritime pine transcriptome. BMC Genomics 12, 366 (2011). https://doi.org/10.1186/1471-2164-12-366
NO AcknowledgementsThe authors gratefully acknowledge the computer resources and technical support provided by the Plataforma Andaluza de Bioinformática of the University of Málaga, Spain. This study was supported by the Spanish Ministerio de Ciencia e Innovación [AGL2009-12139-C02-02, BIO2009-07490], the European Union [PLE2009-0016] and the Junta de Andalucía [CVI-6075 and BIO-114].
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO European Commission
NO Junta de Andalucía
DS Docta Complutense
RD 25 feb 2026