RT Journal Article
T1 One Health surveillance—A cross-sectoral detection, characterization, and notification of foodborne pathogens
A1 Lahti, Elina Tast
A1 Karamehmedovic, Nadja
A1 Riedel, Hilde
A1 Blom, Linnea
A1 Boel, Jeppe
A1 Delibato, Elisabetta
A1 Denis, Martine
A1 van Essen-Zandbergen, Alieda
A1 Garcia Fernandez, Aurora
A1 Hendriksen, Rene
A1 Heydecke, Anna
A1 van Hoek, Angela H. A. M.
A1 Huby, Tom
A1 Kwit, Renata
A1 Lucarelli, Claudia
A1 Lundin, Karl
A1 Michelacci, Valeria
A1 Owczarek, Slawomir
A1 Ring, Isaac
A1 Kjeldgaard, Jette Sejer
A1 Sjögren, Ingegerd
A1 Skóra, Milena
A1 Torpdahl, Mia
A1 Ugarte Ruiz, María
A1 Veldman, Kees
A1 Ventola, Eleonora
A1 Zajac, Magdalena
A1 Jernberg, Cecilia
AB Introduction: Several Proficiency Test (PT) or External Quality Assessment (EQA) schemes are currently available for assessing the ability of laboratories to detect and characterize enteropathogenic bacteria, but they are usually targeting one sector, covering either public health, food safety or animal health. In addition to sector-specific PTs/EQAs for detection, cross-sectoral panels would be useful for assessment of the capacity to detect and characterize foodborne pathogens in a One Health (OH) perspective and further improving food safety and interpretation of cross-sectoral surveillance data. The aims of the study were to assess the cross-sectoral capability of European public health, animal health and food safety laboratories to detect, characterize and notify findings of the foodborne pathogens Campylobacter spp., Salmonella spp. and Yersinia enterocolitica, and to develop recommendations for future cross-sectoral PTs and EQAs within OH. The PT/EQA scheme developed within this study consisted of a test panel of five samples, designed to represent a theoretical outbreak scenario.Methods: A total of 15 laboratories from animal health, public health and food safety sectors were enrolled in eight countries: Denmark, France, Italy, the Netherlands, Poland, Spain, Sweden, and the United Kingdom. The laboratories analyzed the samples according to the methods used in the laboratory and reported the target organisms at species level, and if applicable, serovar for Salmonella and bioserotype for Yersinia.Results: All 15 laboratories analyzed the samples for Salmonella, 13 for Campylobacter and 11 for Yersinia. Analytical errors were predominately false negative results. One sample (S. Stockholm and Y. enterocolitica O:3/BT4) with lower concentrations of target organisms was especially challenging, resulting in six out of seven false negative results. These findings were associated with laboratories using smaller sample sizes and not using enrichment methods. Detection of Salmonella was most commonly mandatory to notify within the three sectors in the eight countries participating in the pilot whereas findings of Campylobacter and Y. enterocolitica were notifiable from human samples, but less commonly from animal and food samples.Discussion: The results of the pilot PT/EQA conducted in this study confirmed the possibility to apply a cross-sectoral approach for assessment of the joint OH capacity to detect and characterize foodborne pathogens.
SN 2296-2565
YR 2023
FD 2023-03-08
LK https://hdl.handle.net/20.500.14352/107550
UL https://hdl.handle.net/20.500.14352/107550
LA eng
NO Tast Lahti E, Karamehmedovic N, Riedel H, Blom L, Boel J, Delibato E, Denis M, van Essen-Zandbergen A, Garcia-Fernandez A, Hendriksen R, Heydecke A, van Hoek AHAM, Huby T, Kwit R, Lucarelli C, Lundin K, Michelacci V, Owczarek S, Ring I, Sejer Kjeldgaard J, Sjögren I, Skóra M, Torpdahl M, Ugarte-Ruiz M, Veldman K, Ventola E, Zajac M and Jernberg C (2023) One Health surveillance—A cross-sectoral detection, characterization, and notification of foodborne pathogens. Front. Public Health 11:1129083. doi: 10.3389/fpubh.2023.1129083
NO Author contributionsET, HR, LB, and CJ designed the study and analyzed the results. ET, NK, HR, LB, and CJ drafted the manuscript. All authors collected data, contributed to the interpretation of the results, contributed to the manuscript, and accepted it.Supplementary materialThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpubh.2023. 1129083/full#supplementary-material
NO European Union
NO Polish Ministry of Education and Science
DS Docta Complutense
RD 2 sept 2024