RT Journal Article
T1 Graft-transmissible induction of potato tuberization by the microRNA miR172
A1 Martin, Antoine
A1 Adam, Hélène
A1 Díaz Mendoza, María Mercedes
A1 Żurczak, Marek
A1 González-Schain, Nahuel
A1 Suárez-López, Paula
AB The photoreceptor phytochrome B (PHYB) and the homeodomain protein BEL5 are involved in the response of potato tuber induction to the photoperiod. However, whether they act in the same tuberization pathway is unknown. Here we show the effect of a microRNA, miR172, on this developmental event. miR172 levels are higher under tuber-inducing short days than under non-inductive long days and are upregulated in stolons at the onset of tuberization. Overexpression of this microRNA in potato promotes flowering,accelerates tuberization under moderately inductive photoperiods and triggers tuber formation under long days. In plants with a reduced abundance of PHYB,which tuberize under long days, both BEL5 mRNA and miR172levels are reduced in leaves and increased in stolons. This, together with the presence of miR172 in vascular bundles and the graft transmissibility of its effect on tuberization, indicates that either miR172 might be mobile or it regulates long-distance signals to induce tuberization. Consistent with this, plants overexpressing miR172 show increased levels of BEL5 mRNA, which has been reported to be transmissible through grafts. Furthermore, we identify an APETALA2-like mRNA containing a miR172 binding site, which is downregulated in plants overexpressing miR172 and plants in which PHYB is silenced. Altogether, our results suggest that miR172 probably acts downstream of the tuberization repressor PHYB and upstream of the tuberization promoter BEL5 and allow us to propose a model for the control of tuberization by PHYB, miR172 and BEL5.
PB The Company of Biologists
SN 0950-1991
YR 2009
FD 2009
LK https://hdl.handle.net/20.500.14352/96202
UL https://hdl.handle.net/20.500.14352/96202
LA eng
NO Antoine Martin, Hélène Adam, Mercedes Díaz-Mendoza, Marek Żurczak, Nahuel D. González-Schain, Paula Suárez-López; Graft-transmissible induction of potato tuberization by the microRNA miR172. Development 1 September 2009; 136 (17): 2873–2881. doi: https://doi.org/10.1242/dev.031658
NO This work was funded by the Spanish Ministry of Education and Science (grant BIO2005-00717, cofinanced by the European Regional Development Fund) and the Centre de Referencia en Biotecnologia of the Generalitat de Catalunya. P.S.-L. was supported by the Ramón y Cajal and I3 programs of the Spanish Ministry of Education and Science and M.Z ˙. by a JAE fellowship from the Spanish Scientific Research Council (CSIC).
NO Generalitat de Catalunya
NO Ministerio de Educación y Ciencia (España)
NO European Commission
DS Docta Complutense
RD 18 abr 2025