RT Journal Article
T1 Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
A1 Schiaffarino, Olivia
A1 Valdivieso González, David
A1 García Pérez, Inés M.
A1 Peñalva, Daniel A.
A1 Almendro Vedia, Víctor Galileo
A1 Natale, Paolo
A1 López Montero, Iván
AB The mitochondrion is an essential organelle enclosed by two membranes whose functionalities depend on their very specific protein and lipid compositions. Proteins from the outer mitochondrial membrane (OMM) are specialized in mitochondrial dynamics and mitophagy, whereas proteins of the inner mitochondrial membrane (IMM) have dedicated functions in cellular respiration and apoptosis. As for lipids, the OMM is enriched in glycerophosphatidyl choline but cardiolipin is exclusively found within the IMM. Though the lipid topology and distribution of the OMM and IMM are known since more than four decades, little is known about the interfacial and dynamic properties of the IMM and OMM lipid extracts. Here we build monolayers, supported bilayers and giant unilamellar vesicles (GUVs) of native OMM and IMM lipids extracts from porcine heart. Additionally, we perform a comparative analysis on the interfacial, phase immiscibility and mechanical properties of both types of extract. Our results show that IMM lipids form more expanded and softer membranes than OMM lipids, allowing a better understanding of the physicochemical and biophysical properties of mitochondrial membranes.
PB FRONTIERS MEDIA SA
YR 2022
FD 2022-09-23
LK https://hdl.handle.net/20.500.14352/115838
UL https://hdl.handle.net/20.500.14352/115838
LA eng
NO Schiaffarino O, Valdivieso González D, García-Pérez IM, Peñalva DA, Almendro-Vedia VG, Natale P and López-Montero I (2022), Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties. Front. Mol. Biosci. 9:910936.
NO Comunidad Autónoma de Madrid
NO Ministerio de Ciencia e Innovación
DS Docta Complutense
RD 14 dic 2025