%0 Journal Article
%A Vilas, José Luis
%A Tagare, Hemant D.
%A Vargas Balbuena, Javier
%A Carazo, Jose María
%A Sorzano, Carlos Óscar S.
%T Measuring local-directional resolution and local anisotropy in cryo-EM maps
%D 2020
%U https://hdl.handle.net/20.500.14352/129693
%X The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.
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