RT Journal Article
T1 Measuring local-directional resolution and local anisotropy in cryo-EM maps
A1 Vilas, José Luis
A1 Tagare, Hemant D.
A1 Vargas Balbuena, Javier
A1 Carazo, Jose María
A1 Sorzano, Carlos Óscar S.
AB The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.
PB Nature Research
YR 2020
FD 2020-01-02
LK https://hdl.handle.net/20.500.14352/129693
UL https://hdl.handle.net/20.500.14352/129693
LA eng
NO Vilas, J.L., Tagare, H.D., Vargas, J. et al. Measuring local-directional resolution and local anisotropy in cryo-EM maps. Nat Commun 11, 55 (2020). https://doi.org/10.1038/s41467-019-13742-w
NO © The Author(s) 2020.Supplementary information is available for this paper at https://doi.org/10.1038/s41467019-13742-w.R01GM114051.RGPIN-2018-04813NC-253837
NO Ministerio de Economía y Competividad (España)
NO Comunidad de Madrid
NO National Institutes of Health (US)
NO Natural Sciences and Engineering Research Council of Canada
NO Gouvernement du Québec
NO European Comission
DS Docta Complutense
RD 20 mar 2026