RT Journal Article
T1 B(reath)e water my friend: hydration of pulmonary surfactant layers as analyzed by fourier transform-infrared spectroscopy
A1 Collada Marugán, Ainhoa
A1 Rosa, Antonio Sebastián
A1 Cejas, Jimena del Pilar
A1 Villalba, Araceli
A1 Batllori Badia, Emma
A1 Galindo Izquierdo, Alberto
A1 Cruz Rodríguez, Antonio
A1 Disalvo, Edgardo Aníbal
A1 Pérez Gil, Jesús
A1 Frías, María de los Ángeles
AB Water is essential for sustaining life, as well as the presence of a fully operational pulmonary surfactant (PS) system coating the respiratory surface. In this work, the interconnection between different hydration states and PS membrane structure is explored thanks to the use of ATR-FTIR spectroscopy. Thermotropic phase transitions and hydration level in complex membrane structures formed by two native surfactant systems, derived from animal and human sources, are compared with those found in synthetic mixtures used routinely to mimic PS composition and with re-assembled samples derived from porcine lavages. Native samples exhibited always a faster and higher hydration level than the synthetic ones under comparable conditions, notwithstanding their origin. This hydration degree was found to be crucial in modulating gel to fluid phase transitions. This new insight should be integrated to better comprehend structure-function relationships in PS natural and model membranes, and provides a novel framework to understand permeability and dynamics of alveolar surfactant coatings under physiological conditions.
PB Elsevier
SN 0003-9861
YR 2025
FD 2025-08
LK https://hdl.handle.net/20.500.14352/122770
UL https://hdl.handle.net/20.500.14352/122770
LA eng
NO Collada, A., Rosa, A. S., Cejas, J. d. P., Villalba, A., Batllori-Badia, E., Galindo, A., Cruz, A., Disalvo, E. A., Pérez-Gil, J., & Frías, M. d. l. Á. (2025). B(reath)e water my friend: hydration of pulmonary surfactant layers as analyzed by fourier transform-infrared spectroscopy. Archives of Biochemistry and Biophysics, 770. https://doi.org/10.1016/J.ABB.2025.110474
NO This work was supported with funds from ANPCyT PICT-2020-SERIEA-01500 and by a grant from the Spanish Ministry of Science and Innovation (PID2021-124932OB-100). Part of this work was carried out in Laboratory of Biointerphase and Biomimetic systems (CIBAAL, UNSE-CONICET) during a two months stay of Ainhoa Collada supported by a joint Project UNSE-Complutense University of Madrid, Spain.
NO Ministerio de Ciencia e Innovación (España)
NO Universidad Complutense de Madrid
NO Universidad Nacional de Santiago del Estero (Argentina)
DS Docta Complutense
RD 19 dic 2025