%0 Journal Article
%A Camacho-Alonso, Fabio
%A Martínez-Ortiz, César
%A Plazas-Buendía, Lucía
%A Mercado-Díaz, Ana
%A Vilaplana-Vivo, Carlos
%A Navarro, José Antonio
%A Buendía, Antonio
%A Merino Martín, José Joaquín
%A Martínez-Beneyto, Yolanda
%T Bone union formation in the rat mandibular symphysis using hydroxyapatite with or without simvastatin: effects on healthy, diabetic, and osteoporotic rats
%D 2020
%@ 1432-6981
%U https://hdl.handle.net/20.500.14352/115627
%X AbstractObjective: The objective is to compare new bone formation in critical defects in healthy, diabetic, and osteoporotic rats filled with hydroxyapatite (HA) alone and HA combined with simvastatin (SV).Materials and methods: A total of 48 adult female Sprague-Dawley rats were randomized into three groups (n = 16 per group): Group, 1 healthy; Group 2, diabetics; and Group 3, osteoporotics. Streptozotocin was used to induce type 1 diabetes in Group 2, while bilateral ovariectomy was used to induce osteoporosis in Group 3. The central portion of the rat mandibular symphysis was used as a physiological critical bone defect. In each group, eight defects were filled with HA alone and eight with HA combined with SV. The animals were sacrificed at 4 and 8 weeks, and the mandibles were processed for micro-computed tomography to analyze radiological union and bone mineral density (BMD); histological analysis of the bone union; and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2).Results: In all groups (healthy, diabetics, and osteoporotics), the defects filled with HA + SV presented greater radiological bone union, BMD, histological bone union, and more VEGF and BMP-2 positivity, in comparison with bone defects treated with HA alone.Conclusions: Combined application of HA and SV improves bone regeneration in mandibular critical bone defects compared with application of HA alone in healthy, diabetic, and osteoporotic rats.Clinical relevance: This study might help to patients with osteoporosis or uncontrolled diabetes type 1, but future studies should be done
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