%0 Journal Article
%A Fuente, Hortensia de la
%A Mittelbrunn, María
%A Sánchez-Martín, Lorena
%A Vicente-Manzanares, Miguel
%A Lamana Domínguez, Amalia
%A Pardi, Ruggero
%A Cabañas Gutiérrez, Carlos
%A Sánchez-Madrid, Francisco
%T Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning
%D 2005
%@ 1939-4586
%U https://hdl.handle.net/20.500.14352/93998
%X Initial adhesive contacts between T lymphocytes and dendritic cells (DCs) facilitate recognition of peptide-MHC complexes by the TCR. In this report, we studied the dynamic behavior of adhesion and Ag receptors on DCs during initial contacts with T-cells. Adhesion molecules LFA-1- and ICAM-1,3-GFP as well as MHC class II-GFP molecules were very rapidly concentrated at the DC contact area. Binding of ICAM-3, and ICAM-1 to a lesser extent, to LFA-1 expressed by mature but not immature DC, induced MHC-II clustering into the immune synapse. Also, ICAM-3 binding to DC induced the activation of the Vav1-Rac1 axis, a regulatory pathway involved in actin cytoskeleton reorganization, which was essential for MHC-II clustering on DCs. Our results support a model in which ICAM-mediated MHC-II clustering on DC constitutes a priming mechanism to enhance antigen presentation to T-cells.
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