RT Journal Article
T1 Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–mediated Initial T-Cell Scanning
A1 Fuente, Hortensia de la
A1 Mittelbrunn, María
A1 Sánchez-Martín, Lorena
A1 Vicente-Manzanares, Miguel
A1 Lamana Domínguez, Amalia
A1 Pardi, Ruggero
A1 Cabañas Gutiérrez, Carlos
A1 Sánchez-Madrid, Francisco
AB Initial adhesive contacts between T lymphocytes and dendritic cells (DCs) facilitate recognition of peptide-MHC complexes by the TCR. In this report, we studied the dynamic behavior of adhesion and Ag receptors on DCs during initial contacts with T-cells. Adhesion molecules LFA-1- and ICAM-1,3-GFP as well as MHC class II-GFP molecules were very rapidly concentrated at the DC contact area. Binding of ICAM-3, and ICAM-1 to a lesser extent, to LFA-1 expressed by mature but not immature DC, induced MHC-II clustering into the immune synapse. Also, ICAM-3 binding to DC induced the activation of the Vav1-Rac1 axis, a regulatory pathway involved in actin cytoskeleton reorganization, which was essential for MHC-II clustering on DCs. Our results support a model in which ICAM-mediated MHC-II clustering on DC constitutes a priming mechanism to enhance antigen presentation to T-cells.
PB American Society for Cell Biology
SN 1939-4586
YR 2005
FD 2005
LK https://hdl.handle.net/20.500.14352/93998
UL https://hdl.handle.net/20.500.14352/93998
LA eng
NO De La Fuente, Hortensia, et al. «Synaptic Clusters of MHC Class II Molecules Induced on DCs by Adhesion Molecule–Mediated Initial T-Cell Scanning». Molecular Biology of the Cell, vol. 16, n.o 7, julio de 2005, pp. 3314-22.  https://doi.org/10.1091/mbc.e05-01-0005.
NO Ministerio de Educación, Cultura y Deporte (España)
NO Fundación  Juan March
DS Docta Complutense
RD 9 abr 2025