RT Journal Article
T1 Electrochemical magnetoimmunosensing platform for determination of the milk allergen β-lactoglobulin
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Campuzano Ruiz, Susana
A1 Conzuelo, Felipe
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Gamella Carballo, María
A1 Reviejo García, Ángel Julio
A1 Pingarrón Carrazón, José Manuel
AB A very sensitive magnetoimmunosensor for the determination of β-lactoglobulin (β-LG) is reported in this work. A sandwich configuration involving covalent immobilization of the capture antibody (antiβ-LG) onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a horseradish peroxidase labeled antibody (HRP-antiβ-LG), is used. The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as electron transfer mediator and hydrogen peroxide as the enzyme substrate. The β-LG magnetoimmunosensor exhibited a wide range of linearity (2.8–100 ng/mL) and a low detection limit of 0.8 ng/mL (20 pg in 25 μL sample). The magnetoimmunosensing platform was successfully applied for the detection of β-LG in different types of milk without any matrix effect after just a sample dilution. The results correlated properly with those provided by a commercial ELISA method offering a truthful analytical screening tool. These features make the developed methodology a promising alternative in the development of user-friendly devices for on-site determination of β-LG in dairy products.
PB Elsevier BV
SN 0039-9140
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/95450
UL https://hdl.handle.net/20.500.14352/95450
LA eng
NO Ruiz-Valdepeñas Montiel V, Campuzano S, Conzuelo F, Torrente-Rodríguez RM, Gamella M, Reviejo AJ, Pingarrón JM. Electrochemical magnetoimmunosensing platform for determination of the milk allergen β-lactoglobulin. Talanta. 2015 Jan;131:156-62. doi: 10.1016/j.talanta.2014.07.076. Epub 2014 Aug 5. PMID: 25281087.
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 10 abr 2025