RT Journal Article
T1 A rapid and simple UHPLC- DAD method for individual anthocyanin analysis: Optimization and validation for wild Mediterranean berries and fruit samples
A1 Fernández Ruiz, Virginia
A1 Sánchez Mata, María De Cortes
A1 Cámara Hurtado, María De La Montaña
A1 Morales Gómez, Patricia
A1 Niño Vega, Erika Maryori
AB Anthocyanins have gained significant attention for their potent colorant capacity and multifaceted biological activities. As a result, the quantification of anthocyanin content in wild berries and the identification of anthocyanin profiles has become increasingly important, as well as the optimization of methods for these determinations. The present study shows a rapid UHPLC-DAD method for identifying and quantifying individual anthocyanins in selected wild berries and fruit samples of Mediterranean origin, namely, Prunus avium, Fragaria vesca and Vaccinium myrtillus. Moreover, the applicability of the developed method to different food matrices was verified in cultivated berries, specifically, Punica granatum, Fragaria x ananassa, Rubus spp., and Vaccinium corymbosum. The developed method showed great sensitivity (LOD of 23.92 ng/g, LOQ of 79.73 ng/g), precision (RSD below 2.599 %), and accuracy (recovery percentages of 97.97 and 98.88%). It successfully quantified individual anthocyanins (delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, petunidin-3-O-glucoside, pelargodinin-3-O-glucoside, malvidin-3-O-glucoside) across different berries and fruit samples. The developed methodology allows to conduct routine analysis without the need for mass spectrometry. The range of total anthocyanins quantified in wild berries and fruit samples, was 0.03 to 7.66 mg/g of powdered extract corresponding to Prunus avium L. and Vaccinium corymbosum L., respectively.
PB Springer
YR 2024
FD 2024-12-04
LK https://hdl.handle.net/20.500.14352/131084
UL https://hdl.handle.net/20.500.14352/131084
LA eng
NO Vega EN, Fernández-Ruiz V, Sánchez-Mata MC, et al. A Rapid and Simple UHPLC- DAD Method for Individual Anthocyanin Analysis: Optimization and Validation for Wild Mediterranean Berries and Fruit Samples. Food Anal Methods 2025;18:471–86. https://doi.org/10.1007/s12161-024-02726-2
NO Ministerio de Ciencia, Innovación y Universidades
DS Docta Complutense
RD 25 feb 2026