RT Journal Article
T1 Biocatalyst engineering of Thermomyces Lanuginosus lipase adsorbed on hydrophobic supports: Modulation of enzyme properties for ethanolysis of oil in solvent-free systems
A1 Abreu Silveira, Erick
A1 Moreno-Pérez, Sonia
A1 Basso, Alessandra
A1 Serban, Simona
A1 Pestana-Mamede, Rita
A1 Tardioli, Paulo
A1 Farinas, Cristiane
A1 Castejón, Natalia
A1 Fernández-Lorente, Gloria
A1 Rocha Martín, Javier
A1 Guisán, José
AB Different immobilized biocatalysts of Thermomyces lanuginosus lipase (TLL) exhibited different properties for the ethanolysis of high oleic sunflower oil in solvent-free systems. TLL immobilized by interfacial adsorption on octadecyl (C-18) supports lost its 1,3-regioselectivity and produced more than 99% of ethyl esters. This reaction was influenced by mass-transfer limitations. TLL adsorbed on macroporous C-18 supports (616 Å of pore diameter) was 10-fold more active than TLL adsorbed on mesoporous supports (100–200 Å of pore diameter) in solvent-free systems. Both derivatives exhibited similar activity when working in hexane in the absence of diffusional limitations. In addition, TLL adsorbed on macroporous Purolite C-18 was 5-fold more stable than TLL adsorbed on mesoporous Sepabeads C-18. The stability of the best biocatalyst was 20-fold lower in anhydrous oil than in anhydrous hexane. Mild PEGylation of immobilized TLL greatly increased its stability in anhydrous hexane at 40 °C, fully preserving the activity after 20 days. In anhydrous oil at 40 °C, PEGylated TLL-Purolite C-18 retained 65% of its initial activity after six days compared to 10% of the activity retained by the unmodified biocatalyst. Macroporous and highly hydrophobic supports (e.g., Purolite C-18) seem to be very useful to prepare optimal immobilized biocatalysts for ethanolysis of oils by TLL in solvent-free systems.
PB Elsevier
SN 0168-1656
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/94108
UL https://hdl.handle.net/20.500.14352/94108
LA eng
NO Abreu Silveira, Erick, et al. «Biocatalyst Engineering of Thermomyces Lanuginosus Lipase Adsorbed on Hydrophobic Supports: Modulation of Enzyme Properties for Ethanolysis of Oil in Solvent-Free Systems». Journal of Biotechnology, vol. 289, enero de 2019, pp. 126-34. https://doi.org/10.1016/j.jbiotec.2018.11.014.
NO FundingEste trabajo contó con el apoyo del Ministerio de Economía, Industria y Competitividad de España (proyectos BIO2012-36861 y CTQ2015-70348). Javier Rocha-Martin agradece la beca Juan de la Cierva (número de subvención IJCI-2014-19260 ) financiada por el Ministerio de Economía, Industria y Competitividad de España . También agradecemos a la Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) por la concesión de la beca a Erick Abreu-Silveira y por las becas 2016/10636-8 , 2015/10530-2 y 2013/20826-0 .
NO Ministerio de Economía, Industria y Competitividad (España)
NO São Paulo Research Foundation (Brazil)
DS Docta Complutense
RD 18 abr 2025