RT Journal Article
T1 Valvular Aortic Stenosis: A Proteomic Insight
A1 Gil Dones, Félix
A1 Martin-Rojas, Tatiana
A1 Lopez-Almodovar, Luis
A1 Cuesta, Fernando de La
A1 Darde, Veronica
A1 Álvarez Llamas, Gloria
A1 Juarez-Tosina, Rocio
A1 Barroso, Gemma
A1 Vivanco, Fernando
A1 Padial, Luis
A1 Barderas, Maria
AB Calcified aortic valve disease is a slowly progressive disorder that ranges from mild valve thickening with no obstruction of blood flow, known as aortic sclerosis, to severe calcification with impaired leaflet motion or aortic stenosis. In the present work we describe a rapid, reproducible and effective method to carry out proteomic analysis of stenotic human valves by conventional 2-DE and 2D-DIGE, minimizing the interference due to high calcium concentrations. Furthermore, the protocol permits the aortic stenosis proteome to be analysed, advancing our knowledge in this area.Until recently, aortic stenosis (AS) was considered a passive process secondary to calcium deposition in the aortic valves. However, it has recently been highlighted that the risk factors associated with the development of calcified AS in the elderly are similar to those of coronary artery disease. Furthermore, degenerative AS shares histological characteristics with atherosclerotic plaques, leading to the suggestion that calcified aortic valve disease is a chronic inflammatory process similar to atherosclerosis. Nevertheless, certain data does not fit with this theory making it necessary to further study this pathology. The aim of this study is to develop an effective protein extraction protocol for aortic stenosis valves such that proteomic analyses can be performed on these structures. In the present work we have defined a rapid, reproducible and effective method to extract proteins and that is compatible with 2-DE, 2D-DIGE and MS techniques. Defining the protein profile of this tissue is an important and challenging task that will help to understand the mechanisms of physiological/pathological processes in aortic stenosis valves.
PB SAGE Publications
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/94763
UL https://hdl.handle.net/20.500.14352/94763
LA eng
NO Gil-Dones F, Martin-Rojas T, Lopez-Almodovar LF, et al. Valvular Aortic Stenosis: A Proteomic Insight. Clinical Medicine Insights: Cardiology. 2010;4. doi:10.4137/CMC.S3884
NO This work was supported by grants from the Instituto de Salud Carlos III (FIS PI070537 and FIS P080970), Fondo de Investigación Sanitaria de Castilla la Mancha (FISCAM PI2008/08) and CAM (Biomarkers S2006/GEN-0247). G. Alvarez-Llamas is a recipient of a research contract from the “Juan de la Cierva” program (JCI-2006-3349) of the Spanish Ministry of Science and Innovation. F. de la Cuesta was supported by the FIS (FI06/00583) and Tatiana Martin-Rojas was supported by Instituto de Salud Carlos III (RD06/0014/1015–RECAVA).
NO Aortic stenosis
NO Instituto de Salud Carlos III
NO Castilla la Mancha
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 6 oct 2024