RT Journal Article
T1 Role of pulmonary surfactant protein Sp-C dimerization on membrane fragmentation: an emergent mechanism involved in lung defense and homeostasis
A1 Barriga Torrejón, Alejandro
A1 Morán Lalangui, Michelle
A1 Castillo-Sánchez, José Carlos
A1 Mingarro, Ismael
A1 Pérez-Gil, Jesús
A1 García Álvarez, María Begoña
AB Surfactant protein C (SP-C) is a protein present in the pulmonary surfactant system that is involved in the biophysical properties of this lipoprotein complex, but it also has a role in lung defense and homeostasis. In this article, we propose that the link between both functions could rely on the ability of SP-C to induce fragmentation of phospholipid membranes and generate small vesicles that serve as support to present different ligands to cells in the lungs. Our results using bimolecular fluorescence complementation and tunable resistive pulse sensing setups suggest that SP-C oligomerization could be the triggering event that causes membrane budding and nanovesiculation. As shown by fluorescence microscopy and flow cytometry, these vesicles are differentially assimilated by alveolar macrophages and alveolar type II cells, indicating distinct roles of these alveoli-resident cells in the processing of the SP-C- induced vesicles and their cargo. These results depict a more accurate picture of the mechanisms of this protein, which could be relevant for the comprehension of pulmonary pathologies and the development of new therapeutic approaches.
PB Elsevier
SN 0005-2736
YR 2021
FD 2021-02-04
LK https://hdl.handle.net/20.500.14352/8184
UL https://hdl.handle.net/20.500.14352/8184
LA eng
NO Ministerio de Ciencia e Innovación y Universidades (MICINN)
NO Comunidad de Madrid
NO Universidad Complutense de Madrid (UCM)
DS Docta Complutense
RD 9 abr 2025