RT Journal Article
T1 Hepatic proliferation in Gunn rats transplanted with hepatocytes: effect of retrorsine and tri-iodothyronine
A1 Cubero Palero, Francisco Javier
A1 Maganto, P
A1 Mula, N
A1 Ortiz González, Arturo
A1 García Barrutia, María Del Socorro
A1 Codesal, F.J.
A1 Arahuetes Portero, Rosa María
AB Hepatocyte transplantation would offer an attractive alternative to liver transplantation in the treatment of inborn errors of liver metabolism. However, a major problem in most transplantation studies to date has been the limited growth of transplanted cells in the recipient organ. We performed a strategy for selective proliferation of transplanted cells by interfering with the proliferative capacity of resident hepatocytes, using the pyrrolizidine alkaloid retrorsine and then transplanting liver cells in conjunction with repeated administration of triiodothyronine, an inducer of hepatocyte proliferation in rats. In the present study, foetal and adult syngeneic hepatocyte transplantation into spleen was performed in retrorsine-treated hyperbilirubinemic Gunn rats. In parallel, repeated injections of triiodothyronine were given to recipients. Rats were sacrificed at 1, 7, 30 and 90 days after transplantation and blood and bile samples were taken to assess the functionality of transplanted cells. The proliferative activity of transplanted hepatocytes was evaluated using proliferating cell nuclear antigen labelling index. In summary, both adult and foetal hepatocyte transplantation were effective in correcting a metabolic abnormality in Gunn rats for as long as 3 months. The RS/T3 model, as a measure to increase graft function, could represent an important advance to future clinical application of hepatocyte transplantation.
PB Wiley
SN 0960-7722
YR 2005
FD 2005-05-12
LK https://hdl.handle.net/20.500.14352/98129
UL https://hdl.handle.net/20.500.14352/98129
LA eng
NO Cubero FJ, Maganto P, Mula N, Ortiz A, Barrutia MG, Codesal FJ, Arahuetes RM. Hepatic proliferation in Gunn rats transplanted with hepatocytes: effect of retrorsine and tri-iodothyronine. Cell Prolif. 2005 Jun;38(3):137-46.
NO Spanish Social Security Funds for Research
DS Docta Complutense
RD 11 abr 2025