RT Journal Article
T1 Effect of surfactant protein A on the physical properties and surface activity of KL4-surfactant
A1 Sáenz, Alejandra
A1 Cañadas Benito, Olga
A1 Bagatolli, Luís
A1 Sánchez Barbero, Fernando
A1 Johnson, Mark
A1 Casals Carro, María Cristina
AB SP-A, the major protein component of pulmonary surfactant, is absent in exogenous surfactants currently used in clinical practice. However, it is thought that therapeutic properties of natural surfactants improve after enrichment with SP-A. The objective of this study was to determine SP-A effects on physical properties and surface activity of a new synthetic lung surfactant based on a cationic and hydrophobic 21-residue peptide KLLLLKLLLLKLLLLKLLLLK, KL4. We have analyzed the interaction of SP-A with liposomes consisting of DPPC/POPG/PA (28:9:5.6, w/w/w) with and without 0.57 mol % KL4 peptide. We found that SP-A had a concentration-dependent effect on the surface activity of KL4-DPPC/POPG/PA membranes but not on that of an animal-derived LES. The surface activity of KL4-surfactant significantly improved after enrichment with 2.5–5 wt % SP-A. However, it worsened at SP-A concentrations $10 wt %. This was due to the fluidizing effect of supraphysiological SP-A concentrations on KL4-DPPC/POPG/PA membranes as determined by fluorescence anisotropy measurements, calorimetric studies, and confocal fluorescence microscopy of GUVs. High SP-A concentrations caused disappearance of the solid/fluid phase coexistence of KL4-surfactant, suggesting that phase coexistence might be important for the surface adsorption process.
PB Elsevier
SN 0006-3495
YR 2007
FD 2007
LK https://hdl.handle.net/20.500.14352/93174
UL https://hdl.handle.net/20.500.14352/93174
LA eng
NO Sáenz A, Cañadas O, Bagatolli LA, Sánchez-Barbero F, Johnson ME, Casals C. Effect of surfactant protein A on the physical properties and surface activity of KL4-surfactant.  Biophys. J. 2007 Jan 15; 92(2): 482-492. 2007
NO Ministerio de Sanidad (España)
NO Laboratorios Dr. Esteve, S. A. (Barcelona)
NO Norwegian Academy of Natural Sciences Research Council
DS Docta Complutense
RD 11 abr 2025