RT Journal Article
T1 Ghrelin Facilitates GLUT2-, SGLT1- and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)
A1 Blanco Imperiali, Ayelén M.
A1 Bertucci, Juan I.
A1 Ramesh, Naresh
A1 Delgado Saavedra, María Jesús
A1 Valenciano González, Ana Isabel
A1 Unniappan, Suraj
AB Glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. This study aimed to determine whether ghrelin, a multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish (Carassius auratus). Three intestinal glucose transporters, the facilitative glucose transporter 2 (GLUT2), and the sodium/glucose co-transporters 1 (SGLT1) and 2 (SGLT2), were studied. Immunostaining of intestinal sections found colocalization of ghrelin and GLUT2 and SGLT2 in mucosal cells. Some cells containing GLUT2, SGLT1 and SGLT2 coexpressed the ghrelin/growth hormone secretagogue receptor 1a (GHS-R1a). Intraperitoneal glucose administration led to a significant increase in serum ghrelin levels, as well as an upregulation of intestinal preproghrelin, ghrelin O-acyltransferase and ghs-r1 expression. In vivo and in vitro ghrelin treatment caused a concentration- and time-dependent modulation (mainly stimulatory) of GLUT2, SGLT1 and SGLT2. These effects were abolished by the GHS-R1a antagonist [D-Lys3]-GHRP-6 and the phospholipase C inhibitor U73122, suggesting that ghrelin actions on glucose transporters are mediated by GHS-R1a via the PLC/PKC signaling pathway. Finally, ghrelin stimulated the translocation of GLUT2 into the plasma membrane of goldfish primary intestinal cells. Overall, data reported here indicate an important role for ghrelin in the modulation of glucoregulatory machinery and glucose homeostasis in fish.
PB Springer Nature
SN 2045-2322
YR 2017
FD 2017-03-24
LK https://hdl.handle.net/20.500.14352/18018
UL https://hdl.handle.net/20.500.14352/18018
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Ministerio de Educación y Ciencia (MEC)
NO Natural Sciences and Engineering Research Council (NSERC) of Canada
NO Saskatchewan Health Research Foundation (SHRF) (Canada)
NO CIHR
NO Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET) (Argentina)
DS Docta Complutense
RD 2 oct 2024