RT Journal Article
T1 Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor α2 in colorectal cells and tumor tissues with different metastatic potential
A1 Serafín González-Carrato, Verónica
A1 Valverde De La Fuente, Alejandro
A1 Martínez-García, Gonzalo
A1 Martínez-Periñán, Emiliano
A1 Comba, Fausto
A1 Garranzo-Asensio, María
A1 Barderas Manchado, Rodrigo
A1 Yáñez-Sedeño Orive, Paloma
A1 Campuzano Ruiz, Susana
A1 Pingarrón Carrazón, José Manuel
AB In this work, the development of the first integrated electrochemical immunosensor for the determination of the IL-13Rα2 is reported. The strategy involves the immobilization of a biotinylated capture antibody onto streptavidin-modified screen-printed electrodes through grafting with p-aminobezoic acid (p-ABA) and further activation using EDC/Sulfo-NHS chemistry. A hybrid nanomaterial composed of multiwalled carbon nanotubes (MWCNTs) and Graphene Quantum Dots (GQDs) was used as nanocarrier of multiple detector antibody and HRP molecules. Amperometric detection with the system H2O2/hydroquinone (HQ) achieved a linear calibration plot ranging from 2.7 to 100 ng mL−1 IL-13sRα2, with a LOD value of 0.8 ng mL−1. The immunosensor showed an excellent selectivity and was successfully applied to the determination of the target receptor directly in small amounts of raw cellular lysates and extracts of paraffin-embedded tissues from patients diagnosed with colorectal cancer at different stages.
PB Elsevier
SN 0925-4005
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/95375
UL https://hdl.handle.net/20.500.14352/95375
LA eng
NO Serafín V⁠, Valverde A⁠, Martínez-García G⁠, Martínez-Periñán E⁠, Comba F⁠, Garranzo-Asensio M⁠, Barderas R⁠, Yáñez-Sedeño P⁠, Campuzano S⁠, Pingarrón J.M. Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor α2 in colorectal cells and tumor tissues with different metastatic potential.  Sens. Actuators, B. 2019 Enero 3; 284: 711-22
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
NO Instituto de Salud Carlos III
NO European Commission
DS Docta Complutense
RD 4 abr 2025