RT Journal Article
T1 Biotechnological applications of purine and pyrimidine deaminases
A1 Del Arco, Jon
A1 Acosta, Javier
A1 Fernández Lucas, Jesús
AB Deaminases, ubiquitous enzymes found in all living organisms from bacteria to humans, serve diverse and crucial functions. Notably, purine and pyrimidine deaminases, while biologically essential for regulating nucleotide pools, exhibit exceptional versatility in biotechnology. This review systematically consolidates current knowledge on deaminases, showcasing their potential uses and relevance in the field of biotechnology. Thus, their transformative impact on pharmaceutical manufacturing is highlighted as catalysts for the synthesis of nucleic acid derivatives. Additionally, the role of deaminases in food bioprocessing and production is also explored, particularly in purine content reduction and caffeine production, showcasing their versatility in this field. The review also delves into most promising biomedical applications including deaminase-based GDEPT and genome and transcriptome editing by deaminase-based systems. All in all, illustrated with practical examples, we underscore the role of purine and pyrimidine deaminases in advancing sustainable and efficient biotechnological practices. Finally, the review highlights future challenges and prospects in deaminase-based biotechnological processes, encompassing both industrial and medical perspectives.
PB Elsevier
SN 0734-9750
YR 2024
FD 2024-11
LK https://hdl.handle.net/20.500.14352/117062
UL https://hdl.handle.net/20.500.14352/117062
LA eng
NO Del Arco, J., Acosta, J., & Fernández-Lucas, J. (2024). Biotechnological applications of purine and pyrimidine deaminases. Biotechnol. Adv., 77, 108473.
NO Acknowledgements:The authors gratefully acknowledge the financial support provided by the Spanish Ministry of Science and Innovation (grant PID2020-117025RB-I00) and Santander Foundation (grant XSAN192006).
NO Ministerio de Ciencia e Innovación (España)
NO Fundación Santander
DS Docta Complutense
RD 20 abr 2025