RT Journal Article T1 Reproductive cycle stage assessment using non-invasive methods in the Two-Toed Sloth (Choloepus Hoffmanni) A1 Calvo Fernández, Cristina A1 Such, Roger A1 Gómez Redondo, Isabel A1 García Vila, Encarnación A1 Gutiérrez Cepeda, Luna A1 Cerdeira Lozano, Joaquín Vicente A1 Mayenco Aguirre, Ana María A1 Santiago Moreno, Julián A1 Hernández, Leticia A1 Sánchez Calabuig, María Jesús AB The number of two-toed sloths (Choloepus hoffmanni) has significantly decreased in the last years. Deepening the knowledge of this tropical mammal's reproductive physiology is essential to improve captive breeding within conservation programs for this species. However, several aspects of its reproductive biology remain unexplored and have not been described sufficiently. The aim of this work was to describe the estrous cycle and reproductive physiology of two adult female C. hoffmanni by vaginal cytology, appearance of the external genitalia, and behavior. Vaginal cytology assay showed that the average duration of the estrous cycle was 15.1 ± 4.53 d. Positive correlations (P < 0.05) were found between the peak presence of superficial cells (estrous phase) and four parameters: aggressive behavior, pursuing behavior, vulvar swelling, and vaginal discharge. This pilot study, conducted on just two animals, forms a basis for a study design that may be employed for a more comprehensive assessment of the two-toed sloth reproductive physiology and behavior PB BioOne Digital Library SN 1042-7260 YR 2024 FD 2024 LK https://hdl.handle.net/20.500.14352/120004 UL https://hdl.handle.net/20.500.14352/120004 LA eng NO Calvo-Fernandez, C., Such, R., Gutiérrez-Cepeda, L., Gómez-Redondo, I., García-Vila, E., Cerdeira, J., Mayenco-Aguirre, A. M., Santiago-Moreno, J., Hernández, L., & Sánchez-Calabuig, M. J. (2024). REPRODUCTIVE CYCLE STAGE ASSESSMENT IN THE TWO-TOED SLOTH (CHOLOEPUS HOFFMANNI), PRELIMINARY RESULTS. Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians, 54(4), 805–809. https://doi.org/10.1638/2021-0132 DS Docta Complutense RD 19 dic 2025