RT Journal Article
T1 Preparation and characterization of different immobilized and chemically modified preparations of lipase B from Candida antarctica: is it the activation energy a good indicator of the biocatalyst expressed activity?
A1 Hackenhaar, Camila R.
A1 Abellanas Perez, Pedro
A1 Carballares Navarro, Diego
A1 Bolívar Bolívar, Juan Manuel
A1 Rodrigues, Rafael C.
A1 Fernandez Lafuente, Roberto
AB Lipase B from Candida antarctica immobilized on octyl (via interfacial activation) and octyl-vinyl sulfone (covalently attached) agarose beads via different immobilization protocols was submitted to amination and/or glutaraldehyde modifications. The catalytic performance of the resulting biocatalysts significantly varied across different substrates: using octyl-CALB with the double modification, activity increased 3.5 fold versus triacetin and decreased by 5 % using R-methyl mandelate, while using the covalent biocatalyst, activity increase by 2.2 or 20 %, respectively. Similarly, the stability of the biocatalysts —both in absolute and relative terms— was strongly influenced by the inactivation pH and the substrate used for residual activity determination. Under the tested conditions, activity versus substrate concentration followed first-order kinetics up to the substrate solubility limit, preventing the determination of kinetic parameters such as Kcat or Km. Activation energy (Eₐ) for triacetin hydrolysis was also measured for each biocatalyst under different inactivation states. Interestingly, no consistent correlation was found between Eₐ and enzyme activity. Generally, partial inactivation of the biocatalysts increased Eₐ, although some exceptions were observed. These findings suggest that Eₐ alone does not directly correlate with enzymatic activity, highlighting the complex interplay between structural enzyme modifications, substrate used to determine the enzyme activity, and the enzyme catalytic behavior
PB Elsevier
SN 0141-8130
YR 2025
FD 2025-09
LK https://hdl.handle.net/20.500.14352/123832
UL https://hdl.handle.net/20.500.14352/123832
LA eng
NO Camila R. Hackenhaar, Pedro Abellanas-Perez, Diego Carballares, Juan M. Bolivar, Rafael C. Rodrigues, Roberto Fernandez-Lafuente, Preparation and characterization of different immobilized and chemically modified preparations of lipase B from Candida antarctica: is it the activation energy a good indicator of the biocatalyst expressed activity?, International Journal of Biological Macromolecules, Volume 323, Part 2, 2025, 147310, ISSN 0141-8130, https://doi.org/10.1016/j.ijbiomac.2025.147310.
NO Ministerio de Ciencia, Innovación y Universidades
NO Unión Europea
NO Ministerio de Economía, Comercio y Empresa
NO Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brazil (CAPES)
DS Docta Complutense
RD 21 mar 2026