RT Journal Article
T1 Fast and sensitive diagnosis of autoimmune disorders through amperometric biosensing of serum anti-dsDNA autoantibodies
A1 López Ruiz, María Beatriz
A1 Arévalo Pérez, Beatriz
A1 Serafín González-Carrato, Verónica
A1 Sánchez-Paniagua López, Marta
A1 Montero Calle, Ana
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB This work reports the first amperometric biosensor involving the use of neutravidin-functionalized magnetic microbeads (NA-MBs) modified with a biotinylated-anti-dsDNA (b-dsDNA) as efficient magnetic microcarriers to selectively capture anti-dsDNA autoantibodies (IgG, IgA and IgM AAbs) present in the sera of patients with rheumatoid arthritis (RA). Subsequently, the attached anti-dsDNA AAbs are detected with a mixture of conventional HRP-labeled secondary antibodies (HRP-anti-human IgG/IgM/IgA mixture). The biorecognition event is monitored by amperometric transduction using the hydroquinone (HQ)/H2O2 system upon capturing the modified MBs on the surface of screen-printed carbon electrodes (SPCEs). The developed bioplatform exhibits a linear calibration plot ranging from 1 to 200 IU mL−1 with a LOD of 0.3 IU mL−1 for anti-dsDNA AAbs standards. In addition, the biosensor allows performing the determination of the anti-dsDNA AAbs levels directly in 100-times diluted serum samples from patients diagnosed with RA and in just 75 min. The obtained results are in agreement with those provided by an ELISA kit and allow discrimination between positive and negative samples.
SN 0956-5663
YR 2020
FD 2020-07
LK https://hdl.handle.net/20.500.14352/99942
UL https://hdl.handle.net/20.500.14352/99942
LA eng
NO Arévalo B, Serafín V, Sánchez-Paniagua M, Montero-Calle A, Barderas R, López-Ruíz B, et al. Fast and sensitive diagnosis of autoimmune disorders through amperometric biosensing of serum anti-dsDNA autoantibodies. Biosensors and Bioelectronics 2020;160:112233. https://doi.org/10.1016/j.bios.2020.112233.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Ministerio de Economía, Comercio y Empresa (España)
NO Comunidad de Madrid
NO Ministerio de Educación, Cultura y Deporte (España)
DS Docta Complutense
RD 29 sept 2024