RT Journal Article
T1 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
A1 Madrid González, Ricardo
A1 Arca‑Lafuente, Sonia
A1 Casanueva‑Benítez, Cristina
A1 Crespo‑Bermejo, Celia
A1 Lara‑Aguilar, Violeta
A1 Martín‑Carbonero, Luz
A1 Santos, Ignacio de los
A1 Briz, Verónica
AB Hepatitis C virus (HCV) infection remains a global health problem, detected only in the early stages by molecular tests. Molecular tests detect HCV RNA, which is very prone to degradation by ribonucleases, reason why blood samples must be transported and stored at − 20 °C, or even − 70 °C for long-term storage. Flinders Technology Associates (FTA) cards are a useful sampling collecting device for dry blood spot (DBS) storage, especially for low and middle-income countries (LMIC). In this study, we analyzed viral HCV RNA integrity for long-term storage at room temperature compared to − 20 °C using two diferent types of cards for DBS: FTA Classic and 903 Protein Saver cards. For this purpose, DBS were prepared on these cards using blood or plasma samples from HCV infected patients, and samples were analysed by conventional RT-PCR. Our results showed that 903 Protein Saver cards are the best and cheapest alternative for DBS storage at room temperature. In these conditions, we found that HCV RNA integrity lasted for up to 9 months.
PB Nature Research
SN 2045-2322
YR 2022
FD 2022-06-16
LK https://hdl.handle.net/20.500.14352/72532
UL https://hdl.handle.net/20.500.14352/72532
LA eng
NO Comunidad de Madrid
DS Docta Complutense
RD 9 abr 2025