RT Journal Article
T1 Proteomics Unravels Extracellular Vesicles as Carriers of Classical Cytoplasmic Proteins in Candida albicans
A1 Gil Bona, Ana
A1 Lla Palacios, Arancha
A1 Parra Giraldo, Claudia Marcela
A1 Vivando, F.
A1 Nombela, César
A1 Monteoliva Díaz, Lucía
A1 Gil, Concha
AB Different signal transduction pathways mediated by MAP kinases have been described in Candida albicans. These pathways sense different stimuli and, therefore, elaborate specific responses. Hog1 was identified as the MAPK that is primarily involved in stress response and virulence, while Cek1 was more specific to cell wall biogenesis, mating and biofilm formation. In the present work, mutants defective in both pathways have been characterized under osmotic stress. Both routes are required for a full response against high osmotic challenge, since mutants defective in both pathways displayed aberrant morphology, cell polarity defects and abnormal chitin deposition, which correlate with loss of viability and appearance of apoptotic markers. These alterations occurred in spite of proper Hog1 and Cek1 phosphorylation and increased intra-cellular glycerol accumulation. The relevance of both routes in virulence is shown as ssk1 msb2 sho1 opy2 mutants are avirulent in a mouse systemic model of infection and display reduced virulence in the Galleria mellonella model.
PB ACS
SN 1535-3893
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/34766
UL https://hdl.handle.net/20.500.14352/34766
LA eng
NO Comunidad de Madrid
NO Ministerio de Economía y Competitividad (MINECO)
NO REIPI, Spanish Network for the Research in Infectious Diseases
NO ISCIII
DS Docta Complutense
RD 9 abr 2025