RT Journal Article
T1 Modulation of pPS10 host range by DnaA
A1 Maestro García-Donas, María Beatriz
A1 Sanz, Jesús
A1 Faelen, Michel
A1 Couturier, Martine
A1 Diaz-Orejas, Ramón
A1 Fernandez-Tresguerres, Elena
AB Narrow-host-range plasmid pPS10, originally found in Pseudomonas savastanoi, is unable to replicate in other strains such as Escherichia coli. Here, we report that the establishment of pPS10 in E. coli can be achieved by a triple mutation in the dnaA gene of E. coli (dnaA403), leading to Q14amber, P297S and A412V changes in the DnaA host replication protein (DnaA403 mutant). As the E. coli strain used contained double amber suppressor mutations (supE, supF), the amber codon in dnaA403 can be translated into glutamine or tyrosine. Genetic analysis of DnaA proteins containing either the individual changes or their different combinations suggests that the P297S mutation is crucial for the establishment of the pPS10 replicon in E. coli. The data also indicate that the P297S change is toxic to the cell and that the additional mutations in DnaA403 could contribute to neutralize this toxicity. To our knowledge, this work reports the first chromosome mutant described in the literature that allows the host range broadening of a plasmid, highlights the essential role played by DnaA in the establishment of pPS10 replicon in E. coli and provides support for the hypothesis that interactions between RepA and DnaA modulate the establish-ment of pPS10 in that bacteria and probably in other species.
PB Wiley
SN 0950-382X
YR 2002
FD 2002
LK https://hdl.handle.net/20.500.14352/93649.3
UL https://hdl.handle.net/20.500.14352/93649.3
LA eng
NO Maestro, Beatriz, et al. «Modulation of pPS10 Host Range by DnaA». Molecular Microbiology, vol. 46, n.o 1, octubre de 2002, pp. 223-34.
NO Ministerio de Educación y Ciencia (España)
NO Comunidad de Madrid
NO Unión Europea
DS Docta Complutense
RD 7 abr 2025